Browsing by Author "Matos, M."
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- Aqueous humor erythropoietin levels in open-angle glaucoma patients with and without TTR V30M familial amyloid polyneuropathyPublication . Beirão, J.; Moreira, L.; Oliveira, J.; Menéres, M.; Pessoa, B.; Matos, M.; Pinho-Costa, P.; Torres, P.; Beirão, I.Purpose: Glaucoma is the leading cause of irreversible blindness in familial amyloidotic polyneuropathy (FAP) patients. Erythropoietin (EPO) is a cytokine that has been shown to play a role in neuroprotection and is endogenously produced in the eye. EPO levels in the aqueous humor are increased in eyes with glaucoma. In this study, we evaluated the EPO concentration in the aqueous humor of FAP and non-FAP patients, with and without glaucoma. Methods: Undiluted aqueous humor samples were obtained from 42 eyes that underwent glaucoma surgery, phacoemulsification, or vitrectomy. EPO concentration in the aqueous humor and blood were measured using the Immulite 2000 Xpi using an automatic analyzer (Siemens Healthcare Diagnostics). Results: The mean EPO concentration in the aqueous humor of non-FAP glaucoma eyes group 2(75.73±13.25 mU/ml)was significantly higher than non-FAP cataract eyes (17.22±5.33 mU/ml; p<0.001), FAP glaucoma eyes (18.82±10.16 mU/ml; p<0.001), and FAP nonglaucoma eyes (20.62±6.22 mU/ml; p<0.001). There was no statistically significant difference between FAP nonglaucoma eyes versus non-FAP cataract eyes (p = 0.23) and FAP glaucoma eyes versus FAP nonglaucoma eyes (p = 0.29). In the glaucoma groups, there was no correlation between the aqueous humor EPO concentration and the ocular pressure (p = 0.95) and mean deviation (p = 0.41). There was no correlation between the EPO serum concentration and EPO aqueous humor concentration in our patients (p = 0.77). Conclusions: Unlike other glaucomatous patients, FAP patients with glaucoma do not show increased and potentially neuroprotective endocular EPO production in the aqueous humor and may need more aggressive glaucoma management
- Impact of physical exercise training on DNA damage and repair: does gender play a role?Publication . Silva, Ana Inês; Soares, J.P.; Silva, A.M.; Gaivão, I.; Mota, M.P.; Matos, M.Acute physical exercise is associated with an enhanced aerobic metabolism, which can result in an increased formation of reactive oxygen species (ROS). ROS can react with DNA, causing strand breaks and modified bases, namely 8-oxoguanine, one of the most common products of oxidative DNA damage, which is repaired by 8-oxoguanine DNA glycosylase 1 (OGG1). Regular physical exercise is considered as a key component of a healthy lifestyle, and its preventive effect, at least in part, is due to oxidative stress induced adaptation, which has been related with an increase in antioxidant activity and in oxidative damage repair enzymes. Gender-related differences concerning DNA damage and DNA repair have been reported. Therefore, the main purpose of this study was to analyse the effects of 16 weeks of combined physical exercise training on DNA damage and repair, in 26 healthy Caucasian individuals, 14 males and 12 females.