Browsing by Author "Lyoussi, Badiaa"
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- Magnetite nanoparticles functionalized propolis extract as nano-antibiotics against methicillin resistant strains of Staphylococcus aureusPublication . El-Guendouz, Soukaina; Lyoussi, Badiaa; Lourenço, João P.; Rosa Costa, Ana M.; Miguel, Maria G.; Dias, Cristina M.B.; Manhita, Ana C.; Luísa, Jordão; Nogueira, Isabel; Faleiro, Maria LeonorDue to their ability to penetrate planktonic and biofilm organized bacteria , nano-antimicrobial agents have been perceived, in recent years, in almost every domain of life especially in health care for the control of infectious diseases. Magnetite nanoparticles (MNPs) have been evaluated for inhibiting microbial growth and biofilm formation. The aim of this study was to evaluate the effect of Moroccan propolis extract functionalized magnetite nanoparticles as nanoantibiotics against methicillin resistant Staphylococcus aureus (MRSA) growth. Chemical composition of propolis was established by pyrolysis GC-MS method. The fabricated nanostructure was characterized by X-ray Diffraction, Transmission Electron Microscopy, and Fourier Transform-Infrared Spectroscopy. FTIR spectroscopy data allowed to confirm functionalization of the MNPs upon contact with oleic acid (OA). Further functionalization with either propolis extract or chloramphenicol was also confirmed by observation of the corresponding FTIR spectra. When both components were added together to OA-functionalized MNPs, the presence of the former is clear whereas that of the latter is less evident, although possible. The use of MNPs functionalized with propolis and chloramphenicol (positive control) at MIC value against methicillin susceptible S. aureus (MSSA) and MRSA strains inhibited the bacterial growth. After the treatment with functionalized MNPs the cells showed disruption of the cell wall as assessed by transmission electron microscopy (TEM). The use of the combination of propolis and the chloramphenicol antibiotic in free form at minimum inhibitory concentration largely impaired both MSSA and MRSA strains, after 2 h of treatment no viable cells of MRSA 2 and MRSA 16 were recovered.
- Magnetite nanoparticles functionalized with propolis against methicillin resistant strains of Staphylococcus aureusPublication . El-Guendouz, Soukaina; Lyoussi, Badiaa; Lourenço, João P.; Rosa da Costa, Ana M.; Miguel, Maria G.; Barrocas Dias, Cristina; Manhita, Ana; Jordao, Luisa; Nogueira, Isabel; Faleiro, Maria L.Magnetite nanoparticles (MNPs) have been evaluated for inhibiting microbial growth and biofilm for- mation. In this study the effect of the nanocomposite Moroccan propolis extract / MNPs acting against methicillin resistant strains of Staphylococcus aureus (MRSA) was evaluated. Chemical composition of propolis was established by pyrolysis coupled to gas chromatography and mass spectrometry method (pyrolysis GC/MS). MNPs were obtained through the co-precipitation method. The fabricated nanostruc- ture was characterized by X-ray Diffraction (DRX), Transmission Electron Microscopy (TEM), and Fourier Transform-Infrared Spectroscopy (FTIR). TEM of MNPs provided a particle average size of 15 nm, FTIR spectral analysis enabled a fast way of identification of MNPs, attesting the occurrence of the different combinations. The use of MNPs loaded with propolis and the antibiotic chloramphenicol at Minimum Inhibitory Concentration (MIC) value inhib- ited the bacterial growth of MSSA (methicillin susceptible strain of S. aureus ) and MRSA strains. After the treatment with MNPs-OA-P-CLo nanocomposite (MNPs with oleic acid, propolis and chloramphenicol), the disruption of the bacterial cell was observed by TEM. The combination of propolis and chlorampheni- col in free form at MIC value largely impaired both MSSA and MRSA strains as, after 2 h of treatment, no viable cells of MRSA 2 and MRSA 16 were recovered. Hence, the results elucidated a new antibac- terial nanocomposite synthesis, for possible applications as prospective nanoantibacterial agents or drug carriers.