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Browsing by Author "El Guendouzi, Souraya"

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  • In vitro and intracellular antimycobacterial activity of a Bacillus pumilus strain
    Publication . Hassi, Mohammed; David, Suzanna; Haggoud, Abdellatif; El Guendouzi, Souraya; El Ouarti, Asmae; Ibn Souda, Saad; Iraqui, Mohammed
    Despite the declaration of tuberculosis (TB) as a global emergency by the world health organization (WHO) about 20 years ago, the worldwide problem of this disease has worsened due to increased drug resistance of tuberculosis bacilli and acquired immune deficiency syndrome (AIDS) pandemic. Consequently, fight against multidrug and extensively drug-resistant TB is a high priority for public health and research. The present work describes the isolation of a Bacillus pumilus strain secreting a metabolite of protein nature capable of inhibiting mycobacterial growth (Mycobacterium smegmatis, Mycobacterium aurum and Mycobacterium bovis BCG). This metabolite is not toxic, accumulates within the macrophage and inactivates the bacilli with a comparable efficiency to that of the pure commercial antimycobacterial substance Amikacin.
    2012-01-25Journal article Open access Show more
  • Isolation and identification of Bacillus strains with antimycobacterial activity
    Publication . El Guendouzi, Souraya; David, Susana; Mohammed, Hassi; Abdellatif, Haggoud; Saad Ibn, Souda; Abdellah, Houari; Mohammed, Iraqui
    Tuberculosis is the principal cause of death worldwide due to an infectious disease. The resurgence of tuberculosis, followed by the increase in prevalence of infections caused by nontuberculous mycobacteria (NTM), as well as the multi-drug resistance of mycobacteria to the majority of currently available antibiotics, have encouraged research for new antimycobacterial agents. Soil and water samples from different Moroccan biotopes, have led to the isolation of four bacterial strains (M, R, G and S), showing an inhibitory effect on mycobacterial growth. This effect was shown to be due to secreted substances in the growth medium. From subsequent analysis it was concluded that these strains produced different active substances. Sequencing of the 16S rRNA showed that these isolates belong to the genus Bacillus. The active substance from isolate M, showed the more important inhibitory effect on mycobacterial growth. It is precipitated with ammonium sulfate and lost all activity when treated with Proteinase K, revealing its protein nature.
    2011-10-09Journal article Open access Show more