Percorrer por autor "Deghmane, Ala-Eddine"
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- Phylogenetic Structure and Comparative Genomics of Multi-National Invasive Haemophilus influenzae Serotype a IsolatesPublication . Topaz, Nadav; Tsang, Raymond; Deghmane, Ala-Eddine; Claus, Heike; Lâm, Thiên-Trí; Litt, David; Bajanca-Lavado, Maria Paula; Pérez-Vázquez, María; Vestrheim, Didrik; Giufrè, Maria; Van Der Ende, Arie; Gaillot, Olivier; Kuch, Alicja; McElligott, Martha; Taha, Muhamed-Kheir; Wang, XinRecent reports have indicated a rise of invasive disease caused by Haemophilus influenzae serotype a (Hia) in North America and some European countries. The whole-genome sequences for a total of 410 invasive Hia isolates were obtained from 12 countries spanning the years of 1998 to 2019 and underwent phylogenetic and comparative genomic analysis in order to characterize the major strains causing disease and the genetic variation present among factors contributing to virulence and antimicrobial resistance. Among 410 isolate sequences received, 408 passed our quality control and underwent genomic analysis. Phylogenetic analysis revealed that the Hia isolates formed four genetically distinct clades: clade 1 (n = 336), clade 2 (n = 13), clade 3 (n = 3) and clade 4 (n = 56). A low diversity subclade 1.1 was found in clade 1 and contained almost exclusively North American isolates. The predominant sequence types in the Hia collection were ST-56 (n = 125), ST-23 (n = 98) and ST-576 (n = 51), which belonged to clade 1, and ST-62 (n = 54), which belonged to clade 4. Clades 1 and 4 contained predominantly North American isolates, and clades 2 and 3 predominantly contained European isolates. Evidence of the presence of capsule duplication was detected in clade 1 and 2 isolates. Seven of the virulence genes involved in endotoxin biosynthesis were absent from all Hia isolates. In general, the presence of known factors contributing to β-lactam antibiotic resistance was low among Hia isolates. Further tests for virulence and antibiotic susceptibility would be required to determine the impact of these variations among the isolates.
- Umrah- and travel-associated meningococcal disease due to multiple serogroup W ST-11 sub-strains pre-Hajj 2024Publication . Lucidarme, Jay; Deghmane, Ala-Eddine; Sharma, Shalabh; Meilleur, Courtney; Eriksson, Lorraine; Mölling, Paula; Claus, Heike; van Sorge, Nina; Bettencourt, Célia; Bajanca-Lavado, Paula; Tsang, Raymond S.W.; Caugant, Dominique A.; Stefanelli, Paola; Neri, Arianna; Tzanakaki, Georgina; Lekshmi, Aiswarya; Campbell, Helen; Clark, Stephen A.; Heymer, Emma J.; Ribeiro, Sonia; Willerton, Laura; Walsh, Lloyd; Bai, Xilian; Lâm, Thiên-Trí; Wagle, Basanta R.; Walia, Vishakh; Howie, Rebecca L.; Neatherlin, John; Rubis, Amy; Vachon, Madhura; McNamara, Lucy A.; Ladhani, Shamez N.; Taha, Muhamed-Kheir; Borrow, RayObjectives: Collectively, the Hajj and Umrah pilgrimages draw > 30 million pilgrims to the Kingdom of Saudi Arabia (KSA) each year. Before Hajj 2024 (14 to 19 June), the meningococcal serogroup W ST-11 complex (W:cc11) Hajj-strain sublineage caused multiple international cases of invasive meningococcal disease (IMD) associated with travel to the Middle East and Asia. Here we identify and characterise the strains responsible. Methods: All Hajj strain sublineage genomes on PubMLST.org underwent core genome MLST comparisons (PubMLST.org). Results: Isolates from 30 cases, across seven countries, formed five phylogenetic clusters within two distinct strains. Travel histories included KSA, other Middle Eastern countries, India, Mauritius, Kenya via Turkey, and no known associated travel. The prevalent strain, representing four clusters, had no African, and limited Middle Eastern, representation. The geo-temporal distribution of available genomes indicated Eastern Europe as a possible source. Conclusions: The rapid expansion of Umrah/travel-related W:cc11 IMD cases in early 2024 was due to multiple strains/sublineages. Despite the involvement of non-KSA travel-destinations, the coincidence of cases with the busy month of Ramadan, and the abrupt cessation during Hajj (when vaccine compliance is maximal), suggest that Umrah was a key driver and highlight the need to reinforce mandatory vaccination whilst maintaining global vigilance.