Browsing by Author "Clemente, L."
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- Antimicrobial drug resistance of Campylobacter spp and Salmonella enterica: national data in food producing animals and food of animal originPublication . Clemente, L.; Correia, I.; Ferreira, E.; Manageiro, V.; Jones-Dias, D.; Albuquerque, T.; Themudo, P.; Rocha, T.; Tavares, A.; Geraldes, M.; Barahona, M.J.; Caniça, M.Campylobacter spp and Salmonella enterica are the two most common causes of bacterial foodborne illnesses in humans in developed countries, being food producing animals one the main reservoirs. Antimicrobial susceptibility testing was determined through Minimum Inhibitory Concentration, in 448 isolates of Campylobacter spp recovered from broiler ceca at slaughter (n=351) and broiler carcasses (n=97); and 1600 isolates of S. enterica feed (n=43) and food products of animal origin (n=527). Screening and identification of beta-lactamase and plasmid-mediated quinolone resistance (PMQR) genes were performed by PCR and sequencing. The highest level of resistance in Campylobacter spp isolates recovered from broilers and carcasses was recorded to ciprofloxacin, followed by tetracycline, erythromycin and streptomycin. Four isolates of Campylobacter coli were resistant to gentamicin.
- Detection of plasmid-mediated colistin-resistance genes in Enterobacteriaceae isolates from food-producing animals and meat. Identification of the novel variant mcr-3, Portugal, 2010-2015.Publication . Clemente, L.; Manageiro, V.; Amaro, A.; Correia, I.; Albuquerque, T.; Themudo, P.; Ferreira, E.; Caniça, M.Following the original report of plasmid-mediated colistin resistance (PMCR) in China, several studies in different countries reported a worldwide distribution of the mcr-1 gene in Enterobacteriaceae. A novel variant, mcr-2, was also detected in colistin-resistant Escherichia coli isolates, from sick calves and piglets in Belgium; since that several other mcr-1 variants has been identified. In this study, we analysed colistin-resistant E. coli and Salmonella enterica isolates from different animal origins, for the presence of PMCR encoding genes. Thus, our aim was to understand the extension of the problem of colistin resistance and PMCR, as colistin is the last resort to treat human infections caused by Gram negative bacteria resistant to all antibiotics, namely carbapenems.