Browsing by Author "Carmo, Susana"
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- Evaluation of the genotoxic and cytotoxic effects of titanium dioxide nanoparticles in human lymphocytesPublication . Silva, Maria João; Carmo, Susana; Tavares, Ana; Louro, HenriquetaTitanium dioxide nanoparticles (TiO2-NP) are being increasingly used in cosmetics and pharmaceutical products. In view of its widespread use, there is a strong demand to evaluate their potential adverse effects to man, particularly their genotoxic effects. Although several studies have already addressed this issue, a conclusion about TiO2-NP safety has not been reached yet. As part of a wider EU Joint Action, this work aims at characterizing the genotoxicity of four TiO2-NP (anatase, rutile, and rutile/anatase), in human peripheral blood lymphocytes (PBL). The cytokinesis-block micronucleus (CBMN) assay (OECD 487) was used to assess both genotoxicity and cytotoxicity, following exposure to several concentrations of each NP (5-250 μg/mL), during 30h. Preliminary data indicate that the anatase and rutile TiO2-NP do not display clear doseresponse genotoxic or cytotoxic effects in PBL. Non-toxic effects had been previously reported in PBL and other cell lines, e.g., CHO and human lung cells, whereas genotoxicity had been found by others in human lymphoblastoid cells and in bronchial epithelial cells. Whether the present findings contribute to demonstrate that TiO2-NP are not genotoxic or rather reflect the insensitivity of PBL to the genotoxicity of these NPs is a question that needs to be clarified in future studies.
- Exposure to second-hand smoke in occupational settings: biomarkers of DNA damage and susceptibilityPublication . Louro, Henriqueta; Carmo, Susana; Vital, Nádia; Gomes, Filomena; Penque, Deborah; Simões, Tânia; Silva, Maria JoãoPortuguese legislation prohibits smoking in most indoor public spaces. However, some restaurants/bars remain, in which smoking is still allowed, representing a potential risk for employees, particularly for chronic respiratory diseases and cancer development. The objectives of this project were to compare the indoor air quality of some restaurants with and without smoking permission and, in their workers, to search for associations between respiratory dysfunctions and biomarkers of exposure, biological effects (DNA and proteome alterations) and susceptibility. Herein, we present data on DNA damage and genetic susceptibility in 37 workers occupationally exposed to environmental tobacco smoke (ETS) and from 44 non-exposed workers. DNA damage was assessed by the comet assay in blood leukocytes and by micronucleus (MN) analysis in buccal cells. DNA repair capacity was evaluated by a challenge of blood cells with EMS (32 µM), preceding the comet assay. Polymorphisms in metabolism (GSTP1, GSTM and GSTT) and DNA-repair genes (hOGG1,XRCC1, XRCC3, and NBS1) were analysed by PCR/RFLP. No significant differences in the MN frequency and in level of DNA damage was observed between ETS and non-ETS groups. However, challenge with EMS resulted in a significantly lower level of DNA breaks in ETS-exposed as compared to non-exposed workers (P<0.0001). Concerning polymorphisms analysis, GSTP1 variant allele carriers showed a decreased frequency of MN in buccal cells, which was not influenced by ETS exposure. No association related to the other polymorphisms was detected. In conclusion, the present data show that blood cells from ETS-exposed workers display a lower mutagenic response to EMS challenge, suggestive of an adaptive response elicited by the previous exposure to low levels of ETS.