Percorrer por autor "Alanio, Alexandre"
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- ECMM CandiReg - A ready to use platform for outbreaks and epidemiological studiesPublication . Koehler, Philipp; Arendrup, Maiken Cavling; Arikan‐Akdagli, Sevtap; Bassetti, Matteo; Bretagne, Stéphane; Klingspor, Lena; Lagrou, Katrien; Meis, Jacques F.; Rautemaa‐Richardson, Riina; Schelenz, Silke; Hamprecht, Axel; Koehler, Felix C.; Kurzai, Oliver; Salmanton‐García, Jon; Vehreschild, Jörg‐Janne; Alanio, Alexandre; Alastruey‐Izquierdo, Ana; Arsic Arsenijevic, Valentina; Gangneux, Jean‐Pierre; Gow, Neil A. R.; Hadina, Suzana; Hamal, Petr; Johnson, Elizabeth; Klimko, Nikolay; Lass‐Flörl, Cornelia; Mares, Mihai; Özenci, Volkan; Papp, Tamas; Roilides, Emmanuel; Sabino, Raquel; Segal, Esther; Talento, Alida Fe; Tortorano, Anna Maria; Verweij, Paul E.; Hoenigl, Martin; Cornely, Oliver A.; European Confederation of Medical Mycology (ECMM)Background: Recent outbreaks of Candida auris further exemplify that invasive Candida infections are a substantial threat to patients and healthcare systems. Even short treatment delays are associated with higher mortality rates. Epidemiological shifts towards more resistant Candida spp. require careful surveillance. Objectives: Triggered by the emergence of C auris and by increasing antifungal resistance rates the European Confederation of Medical Mycology developed an international Candida Registry (FungiScope™ CandiReg) to allow contemporary multinational surveillance. Methods: CandiReg serves as platform for international cooperation to enhance research regarding invasive Candida infections. CandiReg uses the General Data Protection Regulation compliant data platform ClinicalSurveys.net that holds the electronic case report forms (eCRF). Data entry is supported via an interactive macro created by the software that can be accessed via any Internet browser. Results: CandiReg provides an eCRF for invasive Candida infections that can be used for a variety of studies from cohort studies on attributable mortality to evaluations of guideline adherence, offering to the investigators of the 28 ECMM member countries the opportunity to document their cases of invasive Candida infection. CandiReg allows the monitoring of epidemiology of invasive Candida infections, including monitoring of multinational outbreaks. Here, we describe the structure and management of the CandiReg platform. Conclusion: CandiReg supports the collection of clinical information and isolates to improve the knowledge on epidemiology and eventually to improve management of invasive Candida infections. CandiReg promotes international collaboration, improving the availability and quality of evidence on invasive Candida infection and contributes to improved patient management.
- A multicentre external quality assessment: A first step to standardise PCR protocols for the diagnosis of histoplasmosis and coccidioidomycosisPublication . Wilmes, Dunja; Hagen, Ferry; Verissimo, Cristina; Alanio, Alexandre; Rickerts, Volker; Buitrago, Maria JoséBackground: In-house real-time PCR (qPCR) is increasingly used to diagnose the so called endemic mycoses as commercial assays are not widely available. Objectives: To compare the performance of different molecular diagnostic assays for detecting Histoplasma capsulatum and Coccidioides spp. in five European reference laboratories. Methods: Two blinded external quality assessment (EQA) panels were sent to each laboratory that performed the analysis with their in-house assays. Both panels in cluded a range of concentrations of H. capsulatum (n= 7) and Coccidioides spp. (n= 6), negative control and DNA from other fungi. Four laboratories used specific qPCRs, and one laboratory a broad-range fungal conventional PCR (cPCR) and a specific cPCR for H. capsulatum with subsequent sequencing. Results: qPCR assays were the most sensitive for the detection of H. capsulatum DNA. The lowest amount of H. capsulatum DNA detected was 1–4 fg, 0.1 pg and 10 pg for qPCRs, specific cPCR and broad-range cPCR, respectively. False positive results oc curred with high concentrations of Blastomyces dermatitidis DNA in two laboratories and with Emergomyces spp. in one laboratory. For the Coccidioides panel, the lowest amount of DNA detected was 1–16 fg by qPCRs and 10 pg with the broad-range cPCR. One labo ratory reported a false positive result by qPCR with high load of Uncinocarpus DNA Conclusion: All five laboratories were able to correctly detect H. capsulatum and Coccidioides spp. DNA and qPCRs had a better performance than specific cPCR and broad-range cPCR. EQAs may help standardise in-house molecular tests for the so called endemic mycoses improving patient management.