DSA - Apresentações orais em encontros internacionais
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- Assessment of Indoor Environmental Quality in Elderly Care CentersPublication . Cano, Manuela; Nogueira, Susana; Alves, Marta; Papoila, Ana Luísa; Aguiar, Fátima; Rosa, Nuno; Brás, Maria Clementina; Quintas, Maria Carmo; Pinhal, Hermínia; Nogueira, Ana; Proença, Carmo; Teixeira, João P.The aim of this study was to characterize indoor environmental quality in a representative sample of Elderly Care Centers (ECC) in order to associate it with ventilation, health and comfort of elderly people. Indoor air quality (IAQ) parameters and thermal comfort were measured twice, during winter and spring/summer seasons, from 18 Elderly Care Centers (ECC) located in Lisbon, with a total of 116 rooms evaluated. Carbon dioxide and carbon monoxide were monitored during occupation periods using the Indoor Air Quality Meter (TSI, model 7545, USA). Formaldehyde was collected by active sampling on impingers, using personal pumps at an airflow of 1L/min and analyzed according to NIOSH 3500 method, using visible spectrometry (UV4, UNICAM). PM10 and PM2,5 were collected by active sampling on pre-weighted PTFE filters mounted on PM10 and PM2,5 collectors (PEM, SKC), using personal pumps operating at 2L/min, followed by gravimetric analysis for particle mass according to the method IP-10A by SKC (2004). Duplicate samples of total volatile organic compounds were collected on TENAX Tubes (Ref. 25054, Supelco) using SKC personal pumps calibrated to 0.05 L/min and analyzed after thermal desorption according to ISO 16000-part 6 using gas chromatography. Duplicate samples of viable airborne bacteria and fungi were collected using the Microbiological Air Sampler (Merck) and TSA, McK and MEA for total bacteria, Gram-negative bacteria and fungi, respectively. Whole-body thermal comfort evaluation was based on PMV (Predicted Mean Vote) and PPD (Predicted Percentage of Dissatisfied) indices, according to the ISO 7730:2005. Considering the obtained results for environmental indoor quality it is possible to conclude that thermal comfort was not reached in more than 30% of the rooms. In winter, carbon dioxide concentrations were above the reference in 20% of the rooms. PM10 and PM2,5 mean concentrations were above the reference levels in approximately 25% and 30% of the rooms, respectively. Microbiological contamination (total bacteria and fungi) was above the reference levels in more than 35% of the rooms. Indoor environmental quality should be improved by controlling contamination sources, ventilation and thermal parameters (or clothing) in order to obtain healthier environments for the elderly.
- Indoor Air Quality in Portuguese Day Care Centers - ENVIRH ProjectPublication . Cano, Manuela; Nogueira, Susana; Papoila, Ana Luísa; Aguiar, Fátima; Martins, Pedro; Marques, João; Caires, Iolanda; Martins, José; Pedro, Catarina; Paixão, Paulo; Rosado-Pinto, José; Leiria-Pinto, Paula; Aelenei, Daniel; Mendes, Ana; Teixeira, João Paulo; Proença, Carmo; Neuparth, NunoThis paper describes field measurements of indoor air quality (IAQ) parameters, performed in 19 Portuguese Children Day Care Centers (CDCC) during the springtime of 2011 and aims to characterize the indoor environment. The results demonstrate an association between carbon dioxide and bacterial concentrations, which in turn are affected by the number of children present in each classroom. Indoor PM10 concentrations were higher than outdoor levels (I/O ratio>1) and was also found statistically significant association between PM10 concentrations and the type of floor covering materials. These results provide evidence that IAQ is inadequate and, as a consequence, human source contaminants such as bacteria and carbon dioxide accumulate indoors. This study suggests that it is necessary to improve ventilation in order to achieve a healthier indoor environment.
- Indoor microbiological contamination in children day care centres – the ENVIRH studyPublication . Cano, Manuela; Nogueira, Susana; Papoila, Ana Luísa; Aguiar, Fátima; Rosa, Nuno; Mendes, Ana; Teixeira, João Paulo; Proença, Carmo; Neuparth, NunoIn developed countries, after their homes, the children day care center (CDCC) is the place where children spend most of their time, so it is important to understand how environmental factors influence children respiratory health. The aim of ENVIRH (Environment and Health in children day care centres) study is to gather information on indoor environment in CDCC in order to correlate it with both ventilation and children’s health. This paper presents field measurements of indoor microbiological parameters, performed in 19 CDCC during spring and winter and aims to characterize the indoor environment. Bacteria and fungi were collected using a MAS-100 impactor with Trypticase Soy Agar, MacConkey Agar and Malt Extract Agar as culture media for total bacteria, Gram-negative bacteria and fungi, respectively. Concentrations were calculated and fungi were identified. Dust samples were collected on filters using a vacuum cleaner with a DustreamTM collector. Concentration of Der p1 and Der f1 dust mite allergens were assayed separately by ELISA quantitative kit. Most of the CDCC revealed bacterial levels above the reference levels defined by the Portuguese legislation. Nurseries presented lower bacterial concentrations than activity rooms. Indoor fungal concentrations were above outdoor levels in 50% of the studied rooms and the predominant mould genera detected in both seasons were Penicillium and Cladosporium. In spring were obtained higher concentrations of house dust mites in dust with 16% of the rooms surpassing the sensitization threshold.
- Occupational Exposure to biological agents in wastewater treatment plantsPublication . Aguiar, Fátima; Rosa, Nuno; Cano, ManuelaPoor risk assessment is one of the emerging issues related with the occupational exposure to biological agents. The principles for the management of biological risks are described in the Directive 2000/54/EC and employers are responsible for ensuring risk assessment. However, a correct exposure assessment is difficult due to the lack of information on biological risks, lack of validation and harmonization of detection and measurement methods for biological agents. In the mean time it is necessary to assess the risks and to identify higher risk tasks in order to protect workers. Aiming to establish the risks associated with routine tasks, culturable microorganisms were collected using a MAS-100 impactor with Trypticase Soy Agar, MacConkey Agar and Malt Extract Agar as culture media for total bacteria, Gram-negative bacteria and fungi, respectively. The assessment was based on comparison of microorganism concentrations with reference environments taking into account exposure data.
- Tobacco in Portugal:present and future wich path?Publication . Proença, Carmo; Aguiar, Fátima
