Repository logo
 
Publication

Analysis of the translatome by ribosome profiling in colorectal cancer

2016-02-28Conference object Restricted access
Simple item page
dc.contributor.authorSilva, Joana
dc.contributor.authorSantos, Hugo
dc.contributor.authorGama-Carvalho, Margarida
dc.contributor.authorRomão, Luísa
dc.date.accessioned2017-03-07T11:59:30Z
dc.date.embargo2025
dc.date.issued2016-02-28
dc.description.abstractColorectal cancer (CRC) is a serious health problem due to its high incidence and mortality rates despite the major advances in cancer therapeutic approaches. CRC carcinogenesis progression is based in a continuous accumulation of genetic alterations that leads to variations in the overall gene expression profiles. This creates the need for deep analysis of cancer gene expression patterns and, thus, a more reliable understanding of the human proteome to disclose the molecular and cellular pathways as well as the regulatory mechanisms involved in cancer progression. Genome wide analyses of gene expression have so far focused on the abundance of mRNA species as measured either by microarray or, more recently, by RNA deep sequencing. However, neither approach provides information on protein synthesis, an important end point of gene expression. Ribosome profiling is an emerging technique that uses deep sequencing to monitor in vivo translation and provide global and quantitative measurements of translation. It can also reveal unexpected complexity in translation, including the presence of ribosomes outside of classical protein-coding regions of the transcriptome. In this approach, translation is profiled by nuclease footprinting of ribosomes on RNA templates and high-throughput sequencing in order to determine the precise positions of ribosomes on a transcript and its overall density. Ribosome profiling studies have been performed in cancer cell lines, where they showed an increase in overall protein identification and new proteins not yet annotated that possibly were originated from N-terminal extensions or upstream open reading frames (uORFs). The main goal of this project is to determine the changes between the translatome of CRC and normal colorectal cells and the role of such alterations in the tumorigenesis process of CRC cells. For this purpose, we will perform ribosome profiling in normal (NCM460) and CRC (HCT116) cell lines. Bioinformatics and gene ontology analysis of the translated mRNAs will elucidate the main cellular pathways through which the corresponding proteins are involved in CRC progression. Then, we will dissect which of these proteins can interfere and induce cell survival of CRC cells. Furthermore, we aim to analyze the potential contribution of translatable short alternative ORFs (AltORFs) and/or the corresponding peptides towards CRC progression. This information will be crucial to the development of new therapeutic strategies for CRC.pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/4524
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectGenómica Funcional e Estruturalpt_PT
dc.subjectExpressão Génicapt_PT
dc.subjectRibosoma Profilingpt_PT
dc.subjectColorectal Cancerpt_PT
dc.titleAnalysis of the translatome by ribosome profiling in colorectal cancerpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlaceInnsbruck, Austriapt_PT
oaire.citation.titleAdvanced Lecture Course on Systems Biology, 28 fevereiro 2016pt_PT
rcaap.rightsembargoedAccesspt_PT
rcaap.typeconferenceObjectpt_PT

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Retreat BioSYS_Poster_Joana Silva 2016.pdf
Size:
2.2 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DGH - Posters/abstracts em congressos internacionais