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Ten years of external quality assessment (EQA) of Neisseria gonorrhoeae antimicrobial susceptibility testing in Europe elucidate high reliability of data

2019-03-25Journal article Open access
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dc.contributor.authorCole, Michelle J.
dc.contributor.authorQuaye, Nerteley
dc.contributor.authorJacobsson, Susanne
dc.contributor.authorDay, Michaela
dc.contributor.authorFagan, Elizabeth
dc.contributor.authorIson, Catherine
dc.contributor.authorPitt, Rachel
dc.contributor.authorSeaton, Shila
dc.contributor.authorWoodford, Neil
dc.contributor.authorStary, Angelika
dc.contributor.authorPleininger, Sonja
dc.contributor.authorCrucitti, Tania
dc.contributor.authorHunjak, Blaženka
dc.contributor.authorMaikanti, Panayiota
dc.contributor.authorHoffmann, Steen
dc.contributor.authorViktorova, Jelena
dc.contributor.authorBuder, Susanne
dc.contributor.authorKohl, Peter
dc.contributor.authorTzelepi, Eva
dc.contributor.authorSiatravani, Eirini
dc.contributor.authorBalla, Eszter
dc.contributor.authorHauksdóttir, Guðrún Svanborg
dc.contributor.authorRose, Lisa
dc.contributor.authorStefanelli, Paola
dc.contributor.authorCarannante, Anna
dc.contributor.authorPakarna, Gatis
dc.contributor.authorMifsud, Francesca
dc.contributor.authorCassar, Rosann Zammit
dc.contributor.authorLinde, Ineke
dc.contributor.authorBergheim, Thea
dc.contributor.authorSteinbakk, Martin
dc.contributor.authorMlynarczyk-Bonikowska, Beata
dc.contributor.authorBorrego, Maria-José
dc.contributor.authorShepherd, Jill
dc.contributor.authorPavlik, Peter
dc.contributor.authorJeverica, Samo
dc.contributor.authorVazquez, Julio
dc.contributor.authorAbad, Raquel
dc.contributor.authorWeiss, Sabrina
dc.contributor.authorSpiteri, Gianfranco
dc.contributor.authorUnemo, Magnus
dc.date.accessioned2020-05-11T15:57:09Z
dc.date.available2020-05-11T15:57:09Z
dc.date.issued2019-03-25
dc.description.abstractBackground: Confidence in any diagnostic and antimicrobial susceptibility testing data is provided by appropriate and regular quality assurance (QA) procedures. In Europe, the European Gonococcal Antimicrobial Susceptibility Programme (Euro-GASP) has been monitoring the antimicrobial susceptibility in Neisseria gonorrhoeae since 2004. Euro-GASP includes an external quality assessment (EQA) scheme as an essential component for a quality-assured laboratory-based surveillance programme. Participation in the EQA scheme enables any problems with the performed antimicrobial susceptibility testing to be identified and addressed, feeds into the curricula of laboratory training organised by the Euro-GASP network, and assesses the capacity of individual laboratories to detect emerging new, rare and increasing antimicrobial resistance phenotypes. Participant performance in the Euro-GASP EQA scheme over a 10 year period (2007 to 2016, no EQA in 2013) was evaluated. Methods: Antimicrobial susceptibility category and MIC results from the first 5 years (2007-2011) of the Euro-GASP EQA were compared with the latter 5 years (2012-2016). These time periods were selected to assess the impact of the 2012 European Union case definitions for the reporting of antimicrobial susceptibility. Results: Antimicrobial susceptibility category agreement in each year was ≥91%. Discrepancies in susceptibility categories were generally because the MICs for EQA panel isolates were on or very close to the susceptibility or resistance breakpoints. A high proportion of isolates tested over the 10 years were within one (≥90%) or two (≥97%) MIC log2 dilutions of the modal MIC, respectively. The most common method used was Etest on GC agar base. There was a shift to using breakpoints published by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) in the latter 5 years, however overall impact on the validity of results was limited, as the percentage categorical agreement and MIC concordance changed very little between the two five-year periods. Conclusions: The high level of comparability of results in this EQA scheme indicates that high quality data are produced by the Euro-GASP participants and gives confidence in susceptibility and resistance data generated by laboratories performing decentralised testing.pt_PT
dc.description.sponsorshipThe study was funded by the European Centre for Disease Prevention and Control (Framework Contract No. ECDC/2013/015).pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationBMC Infect Dis. 2019 Mar 25;19(1):281. doi: 10.1186/s12879-019-3900-zpt_PT
dc.identifier.doi10.1186/s12879-019-3900-zpt_PT
dc.identifier.issn1471-2334
dc.identifier.urihttp://hdl.handle.net/10400.18/6650
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherBMCpt_PT
dc.relation.publisherversionhttps://bmcinfectdis.biomedcentral.com/articles/10.1186/s12879-019-3900-zpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectDisk Diffusion Antimicrobial Testspt_PT
dc.subjectDrug Resistance, Bacterialpt_PT
dc.subjectEuropept_PT
dc.subjectLaboratoriespt_PT
dc.subjectMicrobial Sensitivity Testspt_PT
dc.subjectNeisseria gonorrhoeaept_PT
dc.subjectQuality Controlpt_PT
dc.subjectReproducibility of Resultspt_PT
dc.subjectInfecções Sexualmente Transmissíveispt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleTen years of external quality assessment (EQA) of Neisseria gonorrhoeae antimicrobial susceptibility testing in Europe elucidate high reliability of datapt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue1pt_PT
oaire.citation.startPage281pt_PT
oaire.citation.titleBMC Infectious Diseasespt_PT
oaire.citation.volume19pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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