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In vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differences

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dc.contributor.authorFonseca, C.
dc.contributor.authorPlanchon, S.
dc.contributor.authorSerra, T.
dc.contributor.authorChander, S.
dc.contributor.authorSaibo, N.J.M.
dc.contributor.authorRenaut, J.
dc.contributor.authorOliveira, M.M.
dc.contributor.authorBatista, Rita
dc.date.accessioned2016-02-16T16:33:25Z
dc.date.available2016-02-16T16:33:25Z
dc.date.issued2015-01
dc.description.abstractIdentification of differences between genetically modified plants and their original counterparts plays a central role in risk assessment strategy. Our main goal was to better understand the relevance of transgene presence, genetic, and epigenetic changes induced by transgene insertion, and in vitro culture in putative unintended differences between a transgenic and its comparator. Thus, we have used multiplex fluorescence 2DE coupled with MS to characterize the proteome of three different rice lines (Oryza sativa L. ssp. japonica cv. Nipponbare): a control conventional line (C), an Agrobacterium-transformed transgenic line (Ta) and a negative segregant (NSb). We observed that Ta and NSb appeared identical (with only one spot differentially abundant--fold difference ≥ 1.5), contrasting with the control (49 spots with fold difference ≥ 1.5, in both Ta and NSb vs. control). Given that in vitro culture was the only event in common between Ta and NSb, we hypothesize that in vitro culture stress was the most relevant condition contributing for the observed proteomic differences. MS protein identification support our hypothesis, indicating that Ta and NSb lines adjusted their metabolic pathways and altered the abundance of several stress related proteins in order to cope with in vitro culture.por
dc.identifier.citationProteomics. 2015 Jan;15(1):124-34. doi: 10.1002/pmic.201400018. Epub 2014 Dec 4por
dc.identifier.doi10.1002/pmic.201400018
dc.identifier.issn1615-9853
dc.identifier.urihttp://hdl.handle.net/10400.18/3342
dc.language.isoengpor
dc.peerreviewedyespor
dc.publisherProteomicspor
dc.relation.publisherversionwww.proteomics-journal.compor
dc.relation.publisherversionhttp://onlinelibrary.wiley.com/doi/10.1002/pmic.201400018/abstract?systemMessage=Wiley+Online+Library+will+be+unavailable+on+Saturday+27th+February+from+09%3A00-14%3A00+GMT+%2F+04%3A00-09%3A00+EST+%2F+17%3A00-22%3A00+SGT+for+essential+maintenance.++Apologies+for+the+inconvenience.
dc.subjectSegurança Alimentarpor
dc.subjectIn vitro culturepor
dc.subjectPlant proteomicspor
dc.titleIn vitro culture may be the major contributing factor for transgenic versus nontransgenic proteomic plant differencespor
dc.typejournal article
dcterms.publisherWiley-VCH Verlag
dspace.entity.typePublication
oaire.citation.endPage134por
oaire.citation.startPage124por
oaire.citation.titleProteomics 2015por
oaire.citation.volume15por
rcaap.rightsclosedAccesspor
rcaap.typearticlepor

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