Repository logo
 
Publication

Assessing the molecular basis of transferable quinolone resistance in Escherichia coli and Salmonella spp. from food-producing animals and food products

2013-12-27Journal article Restricted access
Simple item page
dc.contributor.authorJones-Dias, D.
dc.contributor.authorManageiro, V.
dc.contributor.authorFrancisco, A.P.
dc.contributor.authorMartins, A.P.
dc.contributor.authorDomingues, G.
dc.contributor.authorLouro, D.
dc.contributor.authorFerreira, E.
dc.contributor.authorCaniça, M.
dc.date.accessioned2017-02-17T11:05:21Z
dc.date.available2017-12-28T01:30:12Z
dc.date.issued2013-12-27
dc.description.abstractEnterobacteriaceae resistant to quinolones frequently arise in animals, being easily disseminated through the food-chain. The aim of this study was to investigate the presence of plasmid-mediated quinolone resistance (PMQR) determinants in Salmonella spp. (n=183) and Escherichia coli (n=180) isolates, collected from food-producing animals and food products among swine, poultry, rabbits and cattle. All isolates were subjected to antimicrobial susceptibility testing and molecular screening of PMQR determinants. β-Lactamase-encoding genes, and the quinolone resistance determining region (QRDR) of gyrA, gyrB, parC and parE genes were also investigated in PMQR-positive isolates. Plasmid characterization was performed by conjugation, followed by replicon-typing. Genetic relatedness of PMQR-positive E. coli was examined by Multilocus Sequence Typing, while Salmonella was previously serotyped. The association of mobile genetic elements and PMQR was investigated through PCR mapping assays. Overall, 4.1% (15/363) isolates harbored qnrB2 (n=3), qnrB19 (n=3), and qnrS1 (n=9) genes. All but one isolate presented one to four mutations in QRDR of gyrA or parC genes, which is consistent with the range of MIC values detected (0.19-64 mg/L) for ciprofloxacin; 60% (9/15) of qnr-harboring isolates were non-susceptible to β-lactam antibiotics which was justified by the presence of β-lactamases from TEM (TEM-1, n=8; TEM-135, n=1) and SHV (SHV-108, n=1) families. Analysis of mobile genetic elements revealed that qnr genes were detected nearby relevant genetic elements like intI1, ISEcl2, IS26 and ISCR1 and enclosed in diverse Inc. type plasmids. This study illustrated the existence of Qnr-producing E. coli and Salmonella from food-producing animals, associated to specific mobile elements that might mediate their transference between species and among distinct settings.pt_PT
dc.description.sponsorshipThis study was supported financially by the PTDC/CVT/65713/2006 project from the Fundação para a Ciência e a Tecnologia. D. Jones-Dias has received research funding from grants BRJ/02/DG/2009 from National Institute of Health Dr. Ricardo Jorge, and SFRH/BD/80001/2011 from Fundação para a Ciência e Tecnologia, Lisbon, Portugal. V. Manageiro received research funding from grants SFRH/BD/32578/2006 and SFRH/BPD/77486/2011, and both A.P. Francisco and G. Domingues received funding grants from PTDC/CVT/65713/2006, from Fundação para a Ciência e Tecnologia, Lisbon, Portugal.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationVet Microbiol. 2013 Dec 27;167(3-4):523-31. doi: 10.1016/j.vetmic.2013.08.010. Epub 2013 Aug 22.pt_PT
dc.identifier.doi10.1016/j.vetmic.2013.08.010pt_PT
dc.identifier.issn0378-1135
dc.identifier.urihttp://hdl.handle.net/10400.18/4241
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0378113513004094pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/pt_PT
dc.subjectAnimalspt_PT
dc.subjectAnti-Bacterial Agentspt_PT
dc.subjectBacterial Proteinspt_PT
dc.subjectCattlept_PT
dc.subjectDrug Resistance, Bacterialpt_PT
dc.subjectEscherichia colipt_PT
dc.subjectInterspersed Repetitive Sequencespt_PT
dc.subjectMicrobial Sensitivity Testspt_PT
dc.subjectPlasmidspt_PT
dc.subjectPoultrypt_PT
dc.subjectQuinolonespt_PT
dc.subjectRabbitspt_PT
dc.subjectSalmonellapt_PT
dc.subjectSwinept_PT
dc.subjectbeta-Lactamasespt_PT
dc.subjectFood Microbiologypt_PT
dc.subjectResistência aos Antimicrobianospt_PT
dc.titleAssessing the molecular basis of transferable quinolone resistance in Escherichia coli and Salmonella spp. from food-producing animals and food productspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/3599-PPCDT/PTDC%2FCVT%2F65713%2F2006/PT
oaire.citation.endPage531pt_PT
oaire.citation.startPage523pt_PT
oaire.citation.titleVeterinary Microbiologypt_PT
oaire.citation.volume167(3-4)pt_PT
oaire.fundingStream3599-PPCDT
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
relation.isProjectOfPublication1bb298ca-f838-4ad5-8808-861fd142046d
relation.isProjectOfPublication.latestForDiscovery1bb298ca-f838-4ad5-8808-861fd142046d

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
1-s2.0-S0378113513004094-main.pdf
Size:
546.73 KB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

DDI - Artigos em revistas internacionais