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Cumulus cell DNA damage linked to fertilization success in females with an ovulatory dysfunction phenotype

2024-11-13Research article Open access
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datacite.subject.fosCiências Médicas::Ciências da Saúde
datacite.subject.fosCiências Naturais::Ciências Biológicas
datacite.subject.sdg03:Saúde de Qualidade
dc.contributor.authorRodrigues, Bárbara
dc.contributor.authorSousa, Vanessa
dc.contributor.authorEsteves, Filipa
dc.contributor.authorVale-Fernandes, Emídio
dc.contributor.authorCosta, Solange
dc.contributor.authorSousa, Daniela
dc.contributor.authorBrandão, Raquel
dc.contributor.authorLeal, Carla
dc.contributor.authorPires, Joana
dc.contributor.authorGaivão, Isabel
dc.contributor.authorTeixeira, João Paulo
dc.contributor.authorNogueira, António J.A.
dc.contributor.authorJorge, Paula
dc.date.accessioned2025-04-04T11:42:28Z
dc.date.available2025-04-04T11:42:28Z
dc.date.issued2024-11-13
dc.description.abstractIntracytoplasmic sperm injection (ICSI) is a widely used technique in fertility centers. ICSI success depends on both nuclear and cytoplasmic oocyte maturation. Cumulus cells, which surround the oocytes, play a pivotal role in oocyte competence. However, the significance of DNA damage in cumulus cells as a marker of fertilization success remains largely unexplored. This study aims to investigate the relationship between DNA damage in cumulus cells of females undergoing ICSI, and oocyte competence, with a focus on in vitro fertilization (IVF) outcomes. We employed the alkaline comet assay to assess DNA damage levels (%TDNA) in cumulus cells and whole blood from 22 potentially fertile females and 35 infertile females, including 20 with an ovulatory disfunction phenotype. Our results revealed significant differences between the levels of %TDNA in cumulus cells and blood. Females with an ovulatory dysfunction phenotype exhibited higher levels of %TDNA in cumulus cells compared to potentially fertile females. Additionally, within the group of females with ovulatory dysfunction, a significant correlation was observed between %TDNA levels and the number of oocytes with two pronuclei. Our findings suggest that blood does not accurately reflect DNA damage in cumulus cells, which was correlated with the fertilization success in females with ovulatory dysfunction. High levels of %TDNA in cumulus cells were associated with a higher likelihood of successful fertilization. Moreover, our results imply that low levels of %TDNA may be linked to oocytes that fail to complete maturation and, consequently, do not fertilize (oocytes with zero pronuclei). Further research with larger cohorts is necessary to validate these findings and to explore potential applications in female fertility. However, our study provides evidence that DNA damage in cumulus cells may serve as a valuable biomarker for predicting fertilization success and oocyte competence.eng
dc.description.sponsorshipThis work was supported by national funds: FCT/ESF (Foundation for Science and Technology/European Social Fund) – Project Reference SFRH/BD/136398/2018 and COVID/BD/153204/2023 to B. Rodrigues and FCT/FSE–Project Reference EXPL/BIA-REP/0423/2021—X-EPIFERTILITY. UMIB - Unit for Multidisciplinary Research in Biomedicine is funded by the FCT Portugal (grant numbers UIDB/00215/2020, and UIDP/00215/2020), and ITR - Laboratory for Integrative and Translational Research in Population Health (LA/P/0064/2020). DEFI (Departamento de Ensino, Formação e Investigação) – Reference 2015-DEFI/145/12. Thanks are due to FCT/Ministry of Science, Technology, and Higher Education (MCTES) for the financial support to CESAM (UIDP/50017/2020+UIDB/50017/2020), through national funds. FE work is supported by FCT and ESF through the PhD individual grant UI/BD/150783/2020.
dc.identifier.citationFront Cell Dev Biol. 2024 Nov 13:12:1448733. doi: 10.3389/fcell.2024.1448733. eCollection 2024
dc.identifier.doi10.3389/fcell.2024.1448733
dc.identifier.eissn2296-634X
dc.identifier.pmid39605981
dc.identifier.urihttp://hdl.handle.net/10400.18/10480
dc.language.isoeng
dc.peerreviewedyes
dc.publisherFrontiers Media
dc.relationRole of metabolic profile and FMR1 modifiers in female fertility
dc.relationCOVID/BD/153204/2023
dc.relationUIDB/00215/2020
dc.relationUIDP/00215/2020
dc.relationLA/P/0064/2020
dc.relationUIDP/50017/2020
dc.relationUIDB/50017/2020
dc.relationUI/BD/150783/2020
dc.relation.hasversionhttps://www.frontiersin.org/journals/cell-and-developmental-biology/articles/10.3389/fcell.2024.1448733/full
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectDNA Damage
dc.subjectComet Assay
dc.subjectCumulus Cells
dc.subjectFertilization Success
dc.subjectOvulatory Dysfunction
dc.subjectGenotoxicidade Ambiental
dc.titleCumulus cell DNA damage linked to fertilization success in females with an ovulatory dysfunction phenotypeeng
dc.typeresearch article
dspace.entity.typePublication
oaire.awardTitleRole of metabolic profile and FMR1 modifiers in female fertility
oaire.awardURIhttp://hdl.handle.net/10400.18/10478
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/UI%2FBD%2F150783%2F2020/PT
oaire.citation.startPage1448733
oaire.citation.titleFrontiers in Cell and Developmental Biology
oaire.citation.volume12
oaire.fundingStreamPOR_NORTE
oaire.fundingStreamPOR_NORTE
oaire.versionhttp://purl.org/coar/version/c_970fb48d4fbd8a85
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
relation.isProjectOfPublicationcedada6a-00be-4b33-b9b0-511339e21fd6
relation.isProjectOfPublicationc67e4748-32c2-442b-8b64-0748a80fefe8
relation.isProjectOfPublication.latestForDiscoverycedada6a-00be-4b33-b9b0-511339e21fd6

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