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Quantitative PCR Detection of Enteric Viruses in Wastewater and Environmental Water Sources by the Lisbon Municipality: a Case Study

2020-02-15Artigo científico Acesso aberto
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dc.contributor.authorTeixeira, Pedro
dc.contributor.authorCosta, Sílvia
dc.contributor.authorBrown, Bárbara
dc.contributor.authorSilva, Susana
dc.contributor.authorRodrigues, Raquel
dc.contributor.authorValério, Elisabete
dc.date.accessioned2021-03-05T09:40:21Z
dc.date.available2021-03-05T09:40:21Z
dc.date.issued2020-02-15
dc.description.abstractCurrent regulations and legislation require critical revision to determine safety for alternative water sources and water reuse as part of the solution to global water crisis. In order to fulfill those demands, Lisbon municipality decided to start water reuse as part of a sustainable hydric resources management, and there was a need to confirm safety and safeguard for public health for its use in this context. For this purpose, a study was designed that included a total of 88 samples collected from drinking, superficial, underground water, and wastewater at three different treatment stages. Quantitative Polimerase Chain Reaction (PCR) detection (qPCR) of enteric viruses Norovirus (NoV) genogroups I (GI) and II (GII) and Hepatitis A (HepA) was performed, and also FIB (E. coli, enterococci and fecal coliforms) concentrations were assessed. HepA virus was only detected in one untreated influent sample, whereas NoV GI/ NoV GI were detected in untreated wastewater (100/100%), secondary treated effluent (47/73%), and tertiary treated effluent (33/20%). Our study proposes that NoV GI and GII should be further studied to provide the support that they may be suitable indicators for water quality monitoring targeting wastewater treatment efficiency, regardless of the level of treatment.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationWater. 2020 Feb 15;12:544; doi:10.3390/w12020544pt_PT
dc.identifier.doi10.3390/w12020544pt_PT
dc.identifier.eissn2073-4441
dc.identifier.urihttp://hdl.handle.net/10400.18/7320
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherMDPIpt_PT
dc.relation.publisherversionhttps://www.mdpi.com/2073-4441/12/2/544pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectNoroviruspt_PT
dc.subjectWater Qualitypt_PT
dc.subjectWater Reusept_PT
dc.subjectÁgua e Solopt_PT
dc.subjectDeterminantes da Saúde e da Doençapt_PT
dc.subjectPortugalpt_PT
dc.subjectLisboapt_PT
dc.titleQuantitative PCR Detection of Enteric Viruses in Wastewater and Environmental Water Sources by the Lisbon Municipality: a Case Studypt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.issue2pt_PT
oaire.citation.startPage544pt_PT
oaire.citation.titleWaterpt_PT
oaire.citation.volume12pt_PT
rcaap.embargofctAcesso de acordo com página web do editor da revista.pt_PT
rcaap.rightsopenAccesspt_PT
rcaap.typearticlept_PT

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