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Evaluation of the cytotoxicity and genotoxicity of ingested titanium dioxide nanomaterials in intestinal cells

2019-08-29Conference object Restricted access
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dc.contributor.authorGramacho, Ana Catarina
dc.contributor.authorMartins, Carla
dc.contributor.authorAssunção, Ricardo
dc.contributor.authorGonçalves, Lídia
dc.contributor.authorSimão Bettencourt, Ana
dc.contributor.authorPaula, Alvito
dc.contributor.authorSilva, Maria João
dc.contributor.authorLouro, Henriqueta
dc.date.accessioned2020-06-09T09:58:27Z
dc.date.available2020-06-09T09:58:27Z
dc.date.issued2019-08-29
dc.description.abstractMany products already available, namely in agriculture and food, contain nanomaterials (NMs) and thus human ingestion of these compounds is probable through consumer products or food chain. Although NMs have been extensively investigated in recent years, the studies have generated contradictory results, possibly due to differences in the physicochemical properties of the NMs studied and to other variables in the tested systems. Furthermore, the NMs properties have been recognized as being context-dependent, i.e. can be affected by the surrounding matrix. These secondary features may be potentially more relevant for determining the toxicological outcome. In particular, processes like digestion may modify the NMs characteristics leading to unexpected toxicity in intestine cells. This work aimed to investigate the nano-bio interactions of titanium dioxide NMs (from Joint Research Centre, Ispra) in the context of intestinal tract and digestion processes, to better understand key events that may be linked to an adverse outcome pathway (AOP). In vitro for digestion was simulated and the NMs secondary properties in the intestinal cell moiety were characterized after this process. The cytotoxic and genotoxic effects of digested NMs were determined after the in vitro exposure of human intestinal cells (Caco-2). In addition, the FPG-comet assay was used to analyze oxidative DNA lesions. The digestion products without the NM showed cytotoxic effects above the concentration of 10% in cell culture medium, leading to the need to reduce its concentration below this level. Therefore, initial dose-range studies set a working range of NM concentrations of 0.14 up to 14 µg/ml (0.5-4.5 % of digestion product), which relates also with the predicted levels of exposure of human intestinal cells in real life conditions. Under these conditions, the preliminary results suggest that the tested titanium dioxide NMs do not yield cytotoxic or genotoxic effects upon 24h of exposure of Caco-2 cells, directly after the digestion process. Future studies will investigate the subcellular localization of NMs, integrity of cell junctions, activation of stress signaling pathways and secretion of inflammatory cytokines, to allow an integrated approach to potential adverse effects of the NMs. By elucidating key events elicited by NMs, linking exposure to adverse outcomes, it is expected to contribute to the safety evaluation of NMs within an AOP landscape.pt_PT
dc.description.sponsorshipFunded by FCT/MCTES through national funds (PIDDAC), PTDC/SAU-PUB/29481/2017 and co-funded by UID/BIM/00009/2013 (Centre for Toxicogenomics and Human Health – ToxOmics, FCT), iMed.ULisboa (Pest-UID/DTP/04138/2018) and CESAM (UID/AMB/50017/2019).pt_PT
dc.description.versionN/Apt_PT
dc.identifier.urihttp://hdl.handle.net/10400.18/6969
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.subjectNanomaterialspt_PT
dc.subjectTitanium Dioxidept_PT
dc.subjectEnvironmental Genotoxicitypt_PT
dc.subjectGastrointestinal Tractpt_PT
dc.subjectFood Safetypt_PT
dc.subjectGenotoxicidade Ambientalpt_PT
dc.subjectSegurança Alimentarpt_PT
dc.titleEvaluation of the cytotoxicity and genotoxicity of ingested titanium dioxide nanomaterials in intestinal cellspt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FBIM%2F00009%2F2013/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FDTP%2F04138%2F2013/PT (2018)
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/5876/UID%2FAMB%2F50017%2F2013/PT (2019)
oaire.citation.conferencePlaceTessaloniki, Grécia.pt_PT
oaire.citation.title19th International Symposium on Toxicity Assessment (ISTA2019), 29 August 2019pt_PT
oaire.fundingStream5876
oaire.fundingStream5876
oaire.fundingStream5876
person.familyNameAssunção
person.familyNameDiogo Gonçalves
person.familyNameCampos Simão Bettencourt
person.familyNameAlvito
person.familyNameSilva
person.familyNameLouro
person.givenNameRicardo
person.givenNameLídia Maria
person.givenNameAna Francisca
person.givenNamePaula
person.givenNameMaria Joao
person.givenNameHenriqueta
person.identifier481718
person.identifier125777
person.identifier157627
person.identifier.ciencia-id5B1D-9EB4-E1D4
person.identifier.ciencia-id7211-22BA-86AD
person.identifier.ciencia-id7C1F-F932-33DC
person.identifier.ciencia-id3B14-020B-5FB1
person.identifier.ciencia-id7710-643D-97A3
person.identifier.ciencia-id721D-2BB1-7DB1
person.identifier.orcid0000-0002-4375-0456
person.identifier.orcid0000-0002-6799-2740
person.identifier.orcid0000-0002-8498-5892
person.identifier.orcid0000-0001-8396-1357
person.identifier.orcid0000-0002-6060-0716
person.identifier.orcid0000-0001-9744-7332
person.identifier.ridE-2738-2014
person.identifier.ridB-6977-2014
person.identifier.scopus-author-id56330129500
person.identifier.scopus-author-id8590147800
person.identifier.scopus-author-id55944437600
person.identifier.scopus-author-id6507971479
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typeconferenceObjectpt_PT
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