Using new scanning electron microscopy (SEM) approaches to elucidate bacterial biofilm architecture

Bandeira, Maria; Duarte, Aida; Carvalho, Patricia ALmeida; Jordão, Luisa

Publication

Using new scanning electron microscopy (SEM) approaches to elucidate bacterial biofilm architecture

2016-06Conference object

dc.contributor.author	Bandeira, Maria
dc.contributor.author	Duarte, Aida
dc.contributor.author	Carvalho, Patricia ALmeida
dc.contributor.author	Jordão, Luisa
dc.date.accessioned	2016-07-08T15:49:50Z
dc.date.available	2017-12-31T01:30:12Z
dc.date.issued	2016-06
dc.description.abstract	Healthcare-associated infections (HAI) are a major public health problem being Klebsiella pneumoniae and nontuberculous mycobacteria, both with high antibiotic resistance rates, among their etiological agent. Since biofilme assembly is pointed as one of the mechanisms involved in emergence of antibiotic resistance understanding bacteria organization within the biofilm and the identification of differences between planktonic and sessile forms of bacteria will be a step forward to fight HAI. In the present work we used SEM as a tool to characterize the internal structure of biofilm assembled on different surfaces. For SEM analysis, biofilms were allowed to form either on six-well cell culture plates, silicon or metallic disks placed inside the wells for different incubation periods at 37 °C. The biofilm assembled on the cell culture dish was for both secondary and backscattered electron analysis as described before. Biofilms assembled on silicon disks instead of being sectioned were prepared as metallographic samples, by grinding with grit SIC paper and polishing with diamond particles. Samples were cleaned (70% ethanol), dried with hot air, further coated and analysed. A preliminary study using FIB-SEM has been performed to access the ultrastructure of biofilms assembled on metallic surfaces. The results obtained showed that the same bacteria assembled biofilms with different ratios of biomass and extracellular matrix depending on the surface. SEM performed on thin sections of biofilms is a powerful tool to elucidate biofilm structure allowing the quantification of the major components. FIB-SEM is also a promising tool in this field.	pt_PT
dc.identifier.uri	http://hdl.handle.net/10400.18/3878
dc.language.iso	eng	pt_PT
dc.peerreviewed	yes	pt_PT
dc.rights.uri	http://creativecommons.org/licenses/by/4.0/	pt_PT
dc.subject	Biofilms	pt_PT
dc.subject	Klebsiella pneumoniae	pt_PT
dc.subject	Scanning Electron Microscopy
dc.subject	Agentes Microbianos e Ambiente
dc.title	Using new scanning electron microscopy (SEM) approaches to elucidate bacterial biofilm architecture	pt_PT
dc.type	conference object
dspace.entity.type	Publication
oaire.citation.conferencePlace	Porto, Portugal	pt_PT
oaire.citation.title	Biofilms 7-International Scientific Meeting, 26-28 June 2016	pt_PT
rcaap.rights	embargoedAccess	pt_PT
rcaap.type	conferenceObject	pt_PT