Clemente, LurdesManageiro, VeraJones-Dias, DanielaCorreia, IvoneThemudo, PatriciaAlbuquerque, TeresaGeraldes, MargaridaMatos, FilipaAlmendra, CláudiaFerreira, EugéniaCaniça, Manuela2016-03-222020-01-012015Res Microbiol. 2015 Sep;166(7):574-83. doi: 10.1016/j.resmic.2015.05.007. Epub 2015 Jun 5.0923-2508http://hdl.handle.net/10400.18/3723These results were partially presented at the 3rd ASM Conference on Antimicrobial Resistance in Zoonotic Bacteria and Foodborne Pathogens in Animals, Humans and the Environment, 26e29 June 2012, Aix-la-Provence and at TEMPH (Trends in Environmental Microbiology for Public Health), 18e21 September 2014, Lisbon.The impact of extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC β-lactamases (PMAβs) of animal origin has been a public health concern. In this study, 562 Salmonella enterica and 598 Escherichia coli isolates recovered from different animal species and food products were tested for antimicrobial resistance. Detection of ESBL-, PMAβ-, plasmid-mediated quinolone resistance (PMQR)-encoding genes and integrons was performed in isolates showing non-wild-type phenotypes. Susceptibility profiles of Salmonella spp. isolates differed according to serotype and origin of the isolates. The occurrence of cefotaxime non-wild-type isolates was higher in pets than in other groups. In nine Salmonella isolates, blaCTX-M (n = 4), blaSHV-12 (n = 1), blaTEM-1 (n = 2) and blaCMY-2 (n = 2) were identified. No PMQR-encoding genes were found. In 47 E. coli isolates, blaCTX-M (n = 15), blaSHV-12 (n = 2), blaCMY-2 (n = 6), blaTEM-type (n = 28) and PMQR-encoding genes qnrB (n = 2), qnrS (n = 1) and aac(6')-Ib-cr (n = 6) were detected. To the best of our knowledge, this study is the first to describe the presence of blaCMY-2 (n = 2) and blaSHV-12 (n = 1) genes among S. enterica from broilers in Portugal. This study highlights the fact that animals may act as important reservoirs of isolates carrying ESBL-, PMAβ- and PMQR-encoding genes that might be transferred to humans through direct contact or via the food chain.engResistência aos AntibióticosSalmonella entericaEscherichia coliESBLPMAβPMQRjournal article10.1016/j.resmic.2015.05.007