Lopes de Carvalho, IsabelMilhano, N.Santos, A.S.Almeida, V.Barros, S.C.De Sousa, R.Núncio, M.S.2012-03-162012-03-162008-08Vector Borne Zoonotic Dis. 2008 Aug;8(4):575-91530-3667doi:10.1089/vbz.2007.0245http://hdl.handle.net/10400.18/835A total of 300 Ixodes ricinus ticks were tested by polymerase chain reaction (PCR) for the presence of Borrelia spp., Rickettsia spp., and Anaplasma phagocytophilum. Sequence analysis demonstrated 8 (2.7%) ticks infected with B. lusitaniae, 60 (20%) with Rickettsia spp., and 1 (0.3%) with A. phagocytophilum. Seven (2.3%) ticks were coinfected with B. lusitaniae and Rickettsia spp., 2 (0.6%) with R. monacensis, and 5 (1.7%) with Rickettsia sp. IRS3. The results of this study suggest simultaneous transmission of multiple tick-borne agents on Madeira Island, Portugal.engEstudos de Vectores e Doenças Infecciosasjournal article