Gradíssimo, AnaDelgado, CandidaVerdasca, NunoPista, Angela2013-07-292013-07-292013-05-27Clin Microbiol Infect. 2013 Oct;19(10):E447-50. doi: 10.1111/1469-0691.12233. Epub 2013 May 9.1198-743Xdoi: 10.1111/1469-0691.12233http://hdl.handle.net/10400.18/1690The aim of this work was to assess the value of the physical status of human papillomavirus (HPV) DNA as a disease marker for cervical cancer development in a set of 248 DNA samples previously genotyped as HPV 16 or 18, by calculating the E2/E6 ratio through real-time PCR. There was a significant difference in integration status according to disease grade for both genotypes (p <0.001). Furthermore, especially for HPV 18, determining the DNA physical status could be a useful biomarker in predicting cervical cancer risk development, with a lower E2/E6 ratio clinically associated with the development of a precancerous lesion.engCervical CancerHuman Papillomavirus 18Molecular MarkerPhysical StatusRisk Assessmentjournal article