Sanches Silva, AnaRibeiro, TiagoAlbuquerque, T.G.Paseiro, PerfectoSendón, RaquelBernaldo de Quirós, AnaLópez-Cervantes, JaimeSánchez-Machado, DaliaSoto Valdez, HerlindaAngulo, InmaculadaAurrekoetxea, GoizaneCosta, H.S.2012-07-132012-07-132012J Sep Sci. 2012 Mar;35(5-6):633-401615-9306doi: 10.1002/jssc.201100855http://hdl.handle.net/10400.18/953Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110 ºC, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v).engSegurança AlimentarQuitosanojournal article