Isidro, JoanaSantos, AndreaNunes, AlexandraBorges, VítorSilva, CatarinaVieira, LuísMendes, Aristides L.Serrano, MónicaHenriques, Adriano O.Gomes, João PauloOleastro, Mónica2019-03-202019-03-202018-04Emerg Infect Dis. 2018 Apr;24(4):741-745. doi: 10.3201/eid2404.1700951080-6040http://hdl.handle.net/10400.18/6256We describe imipenem-resistant and imipenem-susceptible clinical isolates of Clostridium difficile ribotype 017 in Portugal. All ribotype 017 isolates carried an extra penicillin-binding protein gene, pbp5, and the imipenem-resistant isolates had additional substitutions near the transpeptidase active sites of pbp1 and pbp3. These clones could disseminate and contribute to imipenem resistance.engAmino Acid SequenceAnti-Bacterial AgentsClostridium difficileEnterocolitis, PseudomembranousGenome, BacterialHumansImipenemMicrobial Sensitivity TestsMutationPhylogenyPortugalWhole Genome SequencingDrug Resistance, BacterialRibotypingInfecções Gastrointestinaisjournal article10.3201/eid2404.170095