Vitorino, LilianaMargos, GabrieleZé-Zé, LíbiaKurtenbach, KlausCollares-Pereira, Margarida2011-09-172011-09-172010-09Ticks Tick Borne Dis. 2010 Sep;1(3):125-8. Epub 2010 Aug 301877-959Xdoi:10.1016/j.ttbdis.2010.07.001http://hdl.handle.net/10400.18/183Plasmid profilesof2Portuguese Borrelia lusitaniae strains, oneisolatedfromahumanpatientandthe other onefroman Ixodes ricinus tick, wereobtainedbypulsed-fieldgelelectrophoresistoevaluatethe plasmid diversityineachstrain.Overall,amaximumof6plasmidsweredetectedthatrangedfrom 19 kbto76kb,revealingcompletelydifferentplasmidprofilesfromthosepreviouslydescribedforother genospecies of B. burgdorferi sensu lato,thecausativeagentsofLymeborreliosis.Theplasmidlocationof the ospA gene wasinvestigatedbyhybridization,allowingitsallocationtotheplasmidof70kbinstead of the54kblinearplasmiddescribedfor B. burgdorferi sensu strictostrains.engLyme borreliosisBorrelia lusitaniaeGenome organizationospAIxodes ricinusEstudos de Vectores e Doenças InfecciosasPlasmid profile analysis of Portuguese Borrelia lusitaniae strainsjournal article