Genetic Background and Expression of the New qepA4 Gene Variant Recovered in Clinical TEM-1- and CMY-2-Producing Escherichia coli

Manageiro, VeraFélix, DavidJones-Dias, DanielaSampaio, Daniel AVieira, LuísSancho, LuísaFerreira, EugéniaCaniça, Manuela2018-03-072018-03-072017-10-09Front Microbiol. 2017 Oct 9;8:1899. doi: 10.3389/fmicb.2017.01899. eCollection 2017.1664-302Xhttp://hdl.handle.net/10400.18/5353A new QepA4 variant was detected in an O86:H28 ST156-fimH38 Escherichia coli, showing a multidrug-resistance phenotype. PAβN inhibition ofqepA4-harboring transconjugant resulted in increase of nalidixic acid accumulation. TheqepA4andcatA1genes were clustered in a 26.0-kp contig matching an IncF-type plasmid, and containing a Tn21-type transposon with multiple mobile genetic elements. This QepA variant is worrisome because these determinants might facilitate the selection of higher-level resistance mutants, playing a role in the development of resistance, and/or confer higher-level resistance to fluoroquinolones in association with chromosomal mutations.engAntibiotic ResistanceQepA4QuinoloneResistanceGenetic CharacterizationPabNWGSResistência aos AntimicrobianosGenetic Background and Expression of the New qepA4 Gene Variant Recovered in Clinical TEM-1- and CMY-2-Producing Escherichia colijournal article10.3389/fmicb.2017.01899