Carvalho, A.S.Molina, H.Matthiesen, Rune2016-05-242016-05-242016-01-08Sci Rep. 2016 Jan 8;6:18826. doi: 10.1038/srep188262045-2322http://hdl.handle.net/10400.18/3818We present a novel data analysis strategy which combined with subcellular fractionation and liquid chromatography-mass spectrometry (LC-MS) based proteomics provides a simple and effective workflow for global drug profiling. Five subcellular fractions were obtained by differential centrifugation followed by high resolution LC-MS and complete functional regulation analysis. The methodology combines functional regulation and enrichment analysis into a single visual summary. The workflow enables improved insight into perturbations caused by drugs. We provide a statistical argument to demonstrate that even crude subcellular fractions leads to improved functional characterization. We demonstrate this data analysis strategy on data obtained in a MS-based global drug profiling study. However, this strategy can also be performed on other types of large scale biological data.engLC-MSProteomicsBiochemistryCell BiologyCancerComputational biology and bioinformaticsChromatography–mass SpectrometryGenomica FuncionalGenómica Funcional e Estruturaljournal articledoi: 10.1038/srep18826.