Torres, Vukosava MilicPopovic, LazarVaz, FatimaPenque, Deborah2016-10-062016-10-062016In: Rueff J, Rodrigues AS (eds.). Cancer Drug Resistance: Overviews and Methods, Springer, 2016: 281-298978-1-4939-3347-1http://hdl.handle.net/10400.18/4026Uncovering mechanisms of unknown pathological mechanisms and body response to applied medication are the drive forces toward personalized medicine. In this post-genomic era, all eyes are tuned to proteomic field, searching for the answers and explanations by investigating the final physiological functional units – proteins and their proteoforms. Development of cutting-edge mass spectrometric technologies and powerful bioinformatics tools, allowed life-science community mining of disease-specific proteins as biomarkers, which are often hidden by high complexity of the samples and/or small abundance. Nowadays, there are several proteomics-based approaches to study the proteome. This chapter focuses on gold standard proteomics strategies and related issues towards candidate biomarker discovery, which may have diagnostic/prognostic as well as mechanistic utility.engGenomica FuncionalMonitorização de DrogasGenómica Funcional e EstruturalCancerChemotherapeutic ResponseDiscovery-based ProteomicsProteomicsTarget-proteomicsbook part