Sanches-Silva, A.Ribeiro, T.Albuquerque, T.G.Paseiro, P.Sendón, R.Bernaldo de Quirós, A.López-Cervantes, J.Sánchez-Machado, D.Soto Valdez, H.Angulo, I.Pardo Aurrekoetxea, G.Costa, H.S.2013-02-122013-02-122012-12-07Biomed Chromatogr. 2012 Dec 7. doi: 10.1002/bmc.2856. [Epub ahead of print]0269-3879ESSN: 1099-0801http://hdl.handle.net/10400.18/1288Nowadays, there is increasing interest in natural antioxidants from food by-products. Astaxanthin is a potent antioxidant and one of the major carotenoids in crustaceans and salmonids. An ultra-high pressure liquid chromatographic method was developed and validated for the determination of astaxanthin in shrimp by-products, and its migration from new packaging materials to food simulants was also studied. The method uses an UPLCW BEH guard-column (2.1 x 5 mm, 1.7mm particle size) and an UPLCW BEH analytical column (2.1 x 50 mm, 1.7mm particle size). Chromatographic separation was achieved using a programmed gradient mobile phase consisting of (A) acetonitrile–methanol (containing 0.05 M ammonium acetate)–dichloromethane (75:20:5, v/v/v) and (B) ultrapure water. This method was evaluated with respect to validation parameters such as linearity, precision, limit of detection, limit of quantification and recovery. Low-density polyethylene films were prepared with different amounts of the lipid fraction of fermented shrimp waste by extrusion, and migration was evaluated into food simulants (isooctane and ethanol 95%, v/v). Migration was not detected under the tested conditions.engAstaxanthinShrimp by-productsMigrationUltra-high Pressure Liquid ChromatographySegurança Alimentarjournal article