David, SusanaSutre, Ana FilipaSanca, ArmandoMané, AlfredoHenriques, VictorPortugal, ClaraSancho, LuísaCardoso, AnaPaixão, EleonoraDuarte, Elsa LeclercLeite, Clarice Queico FujimuraSalem, Julia IgnezAntunes, Abílio2013-03-222013-03-222012-11-01Int J Tuberc Lung Dis. 2012 Nov;16(11):1535-7. doi: 10.5588/ijtld.11.06581027-3719doi: 10.5588/ijtld.11.0658http://hdl.handle.net/10400.18/1532The diagnosis of tuberculosis is seriously hampered in the absence of standard biosafety laboratory facilities for specimen concentration and Mycobacterium tuberculosis culture. Within a laboratory twinning arrangement, heat-fixed direct smear and sediment from 74 bleach-processed and 20 non-processed specimens from Cumura Hospital, Guinea-Bissau, were sent to Lisbon for molecular evaluation of rifampicin resistance. Sequence analysis of a 369 base-pair rpoB locus detected 3.2% (3/94) resistant specimens. To our knowledge, this represents the first report on the molecular analysis of M. tuberculosis from bleach-processed sputum, an alternative to current diagnostic practice in low-resource settings.engMycobacterium TuberculosisrpoBSputum Smear MicroscopySodium HypochloriteDenaturing High-performance liquid chromatography (DHPLC) SequencingInfecções RespiratóriasResistência aos Antimicrobianosjournal article