Viegas, OlgaEsteves, CristinaRocha, JaneteMelo, ArmindoFerreira, Isabel M.P.L.V.O.2021-04-082021-04-082021-09-12Food Chem. 2021 Mar 1;339:128091. doi: 10.1016/j.foodchem.2020.128091. Epub 2020 Sep 12.0308-8146http://hdl.handle.net/10400.18/7668The discovery of melatonin (Mel) in wines triggered a new interest in the paradigm of health benefits and wine consumption, usually ascribed to trans-resveratrol (trans-RSV). In this context, a dispersive liquid-liquid microextraction for the analysis of Mel and trans-RSV in wines by LC-FLD was developed. A 26-1 factorial design was used to identify the significant variables (p < 0.05) and Central Composite Design was used to achieve the optimal conditions: 300 µL of chloroform (extracting solvent), 1500 µL of acetonitrile (disperser solvent) and 1500 mg of NaCl (ionic strength). Excellent linearity (R2 > 0.9999), repeatability (<3.55%), and accuracy (<7.18%) were obtained using a blank matrix and recoveries (>91.9%) using wines. The method was successfully applied to the analyses of Mel (0.63-7.44 ng mL-1) and trans-RSV (169-2616 ng mL-1) in different wine varieties. Comparison with literature point the overall advantages of the new method.Highlights: Novel approach for simultaneous determination of melatonin and trans-resveratrol; The microextraction procedure is optimized by multivariate optimization strategy; Demonstration of the applicability of the final method in real samples; Novel method has potential for rapid analysis of melatonin and trans-resveratrol.engChromatography, High Pressure LiquidFood AnalysisLiquid Phase MicroextractionMelatoninOsmolar ConcentrationResveratrolSolventsTime FactorsWineComposição dos Alimentosjournal article10.1016/j.foodchem.2020.128091