Ziane, HanifaManageiro, VeraFerreira, EugéniaBektache, SoumiaTazir, MohamedCaniça, Manuela2016-03-242020-12-012015J Microbiol Methods. 2015 Dec;119:243-6. doi: 10.1016/j.mimet.2015.11.002. Epub 2015 Nov 4.0167-7012http://hdl.handle.net/10400.18/3730We developed a new sequential multiplex-PCR-based typing scheme (MPBTS) for pneumococcal capsular classification. The serogroup/type of 37 control isolates obtained by the Quellung reaction, MPBTS, and nucleotide sequencing, were fully concordant. The serogroups/types of 75 invasive isolates determined by MPBTS, presented 100% specificity and 96% sensitivity, when compared with the Quellung reaction.engMultiplex PCRPneumococciQuellung ReactionSensitivitySpecificitySerogroup/typeResistência aos Antimicrobianosjournal article10.1016/j.mimet.2015.11.002