Penque, DeborahMarcus, KatrinTorres, Vukosaca Milic2018-03-222021-03-212018-03J Proteomics. 2018 Mar 20;175:1-2. doi: 10.1016/j.jprot.2017.12.011. Epub 2017 Dec 21.1874-3919http://hdl.handle.net/10400.18/5435In this special issue, an updated overview on ‘where we are and where we are going in the area of top-down proteomics’ is given by Fornelli et al., one of the pioneering groups in top-down proteomics led by the proponent head of the term ‘proteoform’ and founder of the international Consortium in Top-Down Proteomics (CTDP), Neil Kelleher. Although being still technologically challenging, the rapid advances over the past years have demonstrated that the identification and characterization of thousands of proteoforms in high-throughput mode is today feasible for intact proteins< 30 kDa under denaturing condition. To further advance, the development of a new generation of instruments and softwares is still necessary, which may happen in the next coming years as predicted by the authors. The high molecular weight capability of native-top down proteomics, used today to characterize purified protein complexes under non-denaturing condition, is one of these technologies that could be innovated for high throughput top-down purposes. The main driver for this breakthrough is undoubtedly the wider acceptance and application of top-down approaches by proteomic researchers. This journey is already under way, as evidenced by the different articles presented in this special issue.engProteomicsBiomarkersGenómica Funcional e EstruturalGenómica Funcionaljournal article10.1016/j.jprot.2017.12.011