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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.18/965

Título: Characterization of maize allergens - MON810 vs. its non-transgenic counterpart.
Autor: Fonseca, Cátia
Planchon, Sébastien
Renaut, Jenny
Oliveira, Maria Margarida
Batista, Rita
Palavras-chave: Maize Allergy
Genetically Modified food safety
Allergen Identification
2-D gel Electrophoresis
Western Blot
Food Safety
Issue Date: 3-Apr-2012
Editora: Elsevier
Citação: J Proteomics. 2012 Apr 3;75(7):2027-37. Epub 2012 Jan 13
Resumo: One of the main concerns about genetically modified foods and their potential impacts on human health is that the introduction of a new/ altered gene may putatively alter the expression of others, namely endogenous allergens. We intended to evaluate, and to compare, using quantitative real time RT-PCR technique, the expression of 5 already known maize allergens (Zea m14, Zea m25, Zea m27kD, 50kD Zein and trypsin inhibitor) in MON 810 vs. its non-transgenic counterpart, throughout seed development (10, 16 and 23 days after pollination). We have shown that none of the tested allergen genes presented differential expression, with statistic significance, along all tested seed development stages, in MON810 vs. its conventional counterpart. We have also used bidimensional gel electrophoresis followed by Western blotting with plasma from two maize allergic subjects to characterize their immunologic responses against MON 810 vs. its non-transgenic control. Immunoreactive spots were characterized by MS. We have identified fourteen new IgE-binding proteins present in both transgenic and non-transgenic maize.
Arbitragem científica: yes
URI: http://hdl.handle.net/10400.18/965
ISSN: 1874-3919
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S187439191200022X
Appears in Collections:DAN - Artigos em revistas internacionais

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