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Title: Ultra-high pressure LC determination of glucosamine in shrimp by-products and migration tests of chitosan films
Authors: Sanches Silva, Ana
Ribeiro, Tiago
Albuquerque, Tânia
Paseiro, Perfecto
Sendón, Raquel
Bernaldo de Quirós, Ana
López-Cervantes, Jaime
Sánchez-Machado, Dalia
Soto Valdez, Herlinda
Angulo, Inmaculada
Aurrekoetxea, Goizane
Costa, Helena
Keywords: Segurança Alimentar
Issue Date: 2012
Publisher: Wiley
Citation: J Sep Sci. 2012 Mar;35(5-6):633-40
Abstract: Chitosan, a multiple applications molecule, was isolated from shrimp by-products by fermentation. The amount of chitosan in the solid fraction of the fermented extract was measured after its conversion in the respective glucosamine units. The procedure includes an acid hydrolysis (110 ºC, 4 h with HCl 8 M) and a derivatization with 9-fluorenylmethyl chloroformate (Fmoc-Cl). Ultra-high-pressure liquid chromatography method was developed and optimized. Excellent peaks resolution was achieved in just 10 min. The method was evaluated in what concerns to validation parameters such as linearity, repeatability, quantification limit, and recovery. Migration tests of films prepared with chitosan were carried out in two simulants: ultrapure water and ethanol 95% (v/v).
Peer review: yes
ISSN: 1615-9306
Publisher Version:;jsessionid=0A563C82E47D9D50EBCB8D5D4C077811.d03t01
Appears in Collections:DAN - Artigos em revistas internacionais

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