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Title: Validation of host-specific Bacteriodales 16S rRNA genes as markers to determine the origin of faecal pollution in Atlantic Rim countries of the European Union
Author: Gawler, Andrew
Beecher, Jean
Brandão, João
Nora, Carroll
Falcão, Leonor
Gourmelon, Michele
Masterson, Bartholomew
Nunes, Baltazar
Rince, Alain
Porter, Jonathan
Thorp, Martin
Walters, Martin
Meijer, Wim
Rodrigues, Raquel
Keywords: Bacteriodales
Microbial Source Tracking
Infecções Sistémicas e Zoonoses
Issue Date: Aug-2007
Publisher: Elsevier
Citation: Water Res. 2007 Aug;41(16):3780-4. Epub 2007 Mar 7
Abstract: The recent implementation of the Revised Bathing Water Directive in the European Union has highlighted the need for development of effective methods to differentiate between sources of faecal contamination. It had previously been shown that amplification of 16S rRNA genes of host-specific cacteriodales species using the HF183F and CF128F primers could be used as markers for human and bovine faecal contamination in the United States. This paper determined the sensitivity and specificity of these markers in four Atlantic Rim countries (France, Ireland, Portugal and the United Kingdom) to evaluate their usefulness in determining the origin of faecal contamination. It was shown that the HF183F marker displayed high sensitivity (80–100%) and specificity (91–100%), and is reliable as an indication of human faecal contamination. The CF128F marker displayed 100% sensitivity in all four countries. However, strong regional variations in specificity (41–96%) were observed, highlighting the need for local validation before this marker is employed in source tracking of faecal contamination.
Peer review: yes
ISSN: 0043-1354
Publisher Version:
Appears in Collections:DDI - Artigos em revistas internacionais

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