Repositório Científico do Instituto Nacional de Saúde >
Departamento de Genética Humana >
DGH - Artigos em revistas internacionais >

Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.18/875

Título: A new function of ROD1 in nonsense-mediated mRNA decay
Autor: Brazão, Tiago França
Demmers, J.
van IJcken, W.
Strouboulis, J.
Fornerod, M.
Romão, Luísa
Grosveld, F.G.
Palavras-chave: Doenças Genéticas
Genómica Funcional e Estrutural
Issue Date: 21-Mar-2012
Editora: Elsevier
Citação: FEBS Lett. 2012 Apr 24;586(8):1101-10. Epub 2012 Mar 21
Resumo: RNA-binding proteins play a crucial role in the post-transcriptional regulation of gene expression. Polypyrimidine tract binding protein (PTB in humans) has been extensively characterized as an important splicing factor, and has additional functions in 3' end processing and translation. ROD1 is a PTB paralog containing four RRM (RNA recognition motif) domains. Here, we discover a function of ROD1 in nonsense-mediated mRNA decay (NMD). We show that ROD1 and the core NMD factor UPF1 interact and co-regulate an extensive number of target genes. Using a reporter system, we demonstrate that ROD1, similarly to UPF1 and UPF2, is required for the destabilization of a known NMD substrate. Finally, we show through RIP-seq that ROD1 and UPF1 associate with a significant number of common transcripts.
Arbitragem científica: yes
URI: http://hdl.handle.net/10400.18/875
ISSN: 014-5793
Versão do Editor: http://ac.els-cdn.com/99F2182F-294D-4EFC-8ADD-3E43FB18B5DB/FinalDownload/DownloadId-CA388036D3AE994DE22B17E2E652CDCD/99F2182F-294D-4EFC-8ADD-3E43FB18B5DB/S0014579312002025/1-s2.0-S0014579312002025-main.pdf?_tid=03033ad3280936bbee217fb38404abe4&acdnat=1341414077_fc59747c105616bebbdcef26b8a8c5a9
Appears in Collections:DGH - Artigos em revistas internacionais

Files in This Item:

File Description SizeFormat
Brazao et al. 2012.pdf1,26 MBAdobe PDFView/Open
Restrict Access. You can request a copy!
Statistics
FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpaceOrkut
Formato BibTex mendeley Endnote Logotipo do DeGóis 

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

  © 2010 www.insa.pt - Todos os direitos reservados | Feedback Ministério da Saúde
Promotores do RCAAP   Financiadores do RCAAP

Fundação para a Ciência e a Tecnologia Universidade do Minho   Governo Português Ministério da Educação e Ciência PO Sociedade do Conhecimento (POSC) Portal oficial da União Europeia