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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.18/583

Título: Tobacco Smoke Occupational Exposure: Biomarkers of Biological Damage
Autor: Simões, Tânia
Milic, Vukosava D.
Pacheco, S.A.
Aguiar, Fátima
Gomes, Filomena
Louro, Henriqueta
Vital, Nádia
Antunes, Susana
Charro, Nuno
Bruno, Alexandre
Vaz, Fátima
Lopes, C.
Marçal, N.
Fragoso, E.
Proença, C.
Sekera, M.
Hagenfeld, Manuela
Silva, Maria João
Almeida, A.B.
Penque, Deborah
Ruivo, P.
Palavras-chave: Environmental Tobbaco Smoke
DNA Damage
Indoor Air Quality
Smoking
Genotoxicidade Ambiental
Ar e Saúde Ocupacional
Biomarkers
Occupational exposure
Chronic Respiratory Diseases
Issue Date: 17-Oct-2011
Editora: Instituto Nacional de Saúde Doutor Ricardo Jorge, IP
Resumo: High concentration of toxic substances emanated from tobacco smoke in entertainment places such as restaurants, bars and nightclubs may compromise indoor air quality (IAQ) generating environments of likelihood health risk. Their employees, particularly those exposed to second-hand smoke, are at increased risk for developing chronic respiratory diseases such as chronic obstructive pulmonary disease (COPD), asthma and lung cancer. Objectives In this work, we aimed at evaluating relationships between occupational ETS exposure, biological damage (DNA or proteome alterations) and putative respiratory dysfunctions. Materials and Methods A group of restaurants located in Lisbon has been studied, in which fine particles (smaller than 2.5µm; PM2.5), indicative of environmental tobacco smoke (ETS) contamination, were measured. After informed consent, workers were evaluated for acute exposure to ETS based on cotinine levels in urine and clinically evaluated for their respiratory health by spirometry measurements and chestpiece auscultation. Effects of ETS exposure on genotoxic lesions were evaluated by measuring DNA/chromosomes breaks in peripheral blood lymphocytes and buccal mucosa cells. Effects of ETS exposure on plasma proteins is being studied using a 2D-DIGE-MALDI-TOF/TOF approach. To achieve that goal, global proteome characterization is being carried based on the same individual plasma samples collected for genotoxic studies and were pooled according to previous criteria. Results Results have confirmed higher respirable particle levels in smoking-designated areas of those entertainment places, indicating an ETS contamination. Leukocytes from ETS-exposed-workers presented lower levels of genotoxic-induced damage in comparison with non-exposed workers, suggesting an ETS-induced stress adaption response in exposed-workers. By proteomics, we are now to investigating those workers for putative alterations on their plasma proteome to provide additional insights on the adaptative response mechanisms that might be activated by ETS exposition. Conclusion Altogether, this study provides information on indoor air quality of Lisbon smoking entertainment places, in particular ETS contamination, and may provide biomarker candidates for occupational ETS-exposure which might precede respiratory diseases on their employees.
Descrição: Work perfomed by Instituto Nacional Saúde Doutor Ricardo Jorge, Laboratório de Análises de Dopagem, Instituto do Desporto de Portugal, Clínica Universitária Pneumologia, Faculdade Medicina Lisboa, Hospital Santa Maria
URI: http://hdl.handle.net/10400.18/583
Appears in Collections:DSA - Posters/abstracts em congressos internacionais
DGH - Posters/abstracts em congressos internacionais

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