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Please use this identifier to cite or link to this item: http://hdl.handle.net/10400.18/554

Title: Role of SHV-β-lactamase variants in resistance of clinical Klebsiella pneumoniae strains to β-lactams in an Algerian hospital
Authors: Ramdani-Bouguessa, Nadjia
Manageiro, Vera
Jones-Dias, Daniela
Ferreira, Eugénia
Tazir, Mohamed
Caniça, Manuela
Keywords: Resistência aos Antimicrobianos
Kinetic Constants
ESBL
Algeria
Issue Date: 2011
Publisher: Society for General Microbiology
Citation: J Med Microbiol. 2011 Jul; 60(7):983-987. Epub 2011 Mar 17
Abstract: Three clinical Klebsiella pneumoniae strains, KpARG74, KpARG220 and KpARG185, isolated from a hospital in Algeria, carried the novel beta-lactamases SHV-98, SHV-99 and SHV-100, respectively, and co-expressed TEM-1 and either CTX-M-3 or CTX-M-15. In contrast, transformed cells possessing the genes for these novel b-lactamases, i.e. EcDH5a-SHV-98, EcDH5a-SHV-99 and EcDH5a-SHV-100, respectively, carried unique sequence features of blaSHV gene variants, enabling oxyimino-cephalosporin susceptibility and confirming that none of the transformants exhibited extended-spectrum b-lactamase (ESBL) properties. SHV-100 is apparently functional, despite differing from the SHV-1 sequence by duplication of 13 amino acids. The SHV-99 enzyme differed from the parental SHV-1 by the amino acid substitution Asp104AGly, which is an important position in the development of the ESBL phenotype in TEM beta-lactamases. This is the first time, to our knowledge, that this mutation has been reported in clinically occurring isolates. Thus, kinetic characterization of the SHV-99 enzyme was performed. The SHV-99 enzyme showed higher affinity (Km of 196 mM), catalytic activity (kcat of 0.5 s-1) and catalytic efficiency (kcat/Km of 0.003 mM-1 s-1) than SHV-1 b-lactamase against aztreonam. These results showed that the neutral glycine at residue 104 increased the affinity of the enzyme to aztreonam, but was unable to develop the ESBL phenotype in SHV enzymes. As the emergence of new threatening combinations of resistance determinants among nosocomial pathogens is further possible, this study has highlighted the need to reverse the spread of initial mutations.
Peer Reviewed: yes
URI: http://hdl.handle.net/10400.18/554
ISSN: 0022-2615
Publisher version: http://www.sciencedirect.com/science/article/pii/S0732889311004792
Appears in Collections:DDI - Artigos em revistas internacionais

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