Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/5212
Título: Genomic structure and insertion sites of Helicobacter pylori prophages from various geographical origins
Autor: Vale, Filipa F.
Nunes, Alexandra
Oleastro, Mónica
Gomes, João P.
Sampaio, Daniel A.
Rocha, Raquel
Vítor, Jorge M. B.
Engstrand, Lars
Pascoe, Ben
Berthenet, Elvire
Sheppard, Samuel K.
Hitchings, Matthew D.
Mégraud, Francis
Vadivelu, Jamuna
Lehours, Philippe
Palavras-chave: Helicobacter pylori
Prophages
Insertion Sites
Geography
Gastrointestinal Infections
Infecções Gastrointestinais
Data: 16-Fev-2017
Editora: Nature Publishing Group
Citação: Sci Rep. 2017 Feb 16;7:42471. doi: 10.1038/srep42471.
Resumo: Helicobacter pylori genetic diversity is known to be influenced by mobile genomic elements. Here we focused on prophages, the least characterized mobile elements of H. pylori. We present the full genomic sequences, insertion sites and phylogenetic analysis of 28 prophages found in H. pylori isolates from patients of distinct disease types, ranging from gastritis to gastric cancer, and geographic origins, covering most continents. The genome sizes of these prophages range from 22.6-33.0 Kbp, consisting of 27-39 open reading frames. A 36.6% GC was found in prophages in contrast to 39% in H. pylori genome. Remarkably a conserved integration site was found in over 50% of the cases. Nearly 40% of the prophages harbored insertion sequences (IS) previously described in H. pylori. Tandem repeats were frequently found in the intergenic region between the prophage at the 3' end and the bacterial gene. Furthermore, prophage genomes present a robust phylogeographic pattern, revealing four distinct clusters: one African, one Asian and two European prophage populations. Evidence of recombination was detected within the genome of some prophages, resulting in genome mosaics composed by different populations, which may yield additional H. pylori phenotypes.
Peer review: yes
URI: http://hdl.handle.net/10400.18/5212
DOI: 10.1038/srep42471
ISSN: 2045-2322 (print)
2045-2322 (online)
Versão do Editor: https://www.nature.com/articles/srep42471
Aparece nas colecções:DGH - Artigos em revistas internacionais
DDI - Artigos em revistas internacionais

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