Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/4746
Título: Toxicity screening of a novel poly(methylmethacrylate)-Eudragit nanocarrier on L929 fibroblasts
Autor: Graça, Diogo
Louro, Henriqueta
Santos, Joana
Dias, Kamila
Almeida, Antonio J
Gonçalves, Lídia
Silva, Maria João
Bettencourt, Ana
Palavras-chave: Nanoparticles Characterization
Nanomedicine
Nanotoxicology
Polymeric Nanocarriers
Genotoxicity
Genotoxicidade Ambiental
Data: 5-Jul-2017
Editora: Elsevier/EUROTOX
Citação: Toxicol Lett. 2017 Jul 5;276:129-137. doi: 10.1016/j.toxlet.2017.05.017. Epub 2017 May 17
Resumo: Translation of innovative drug delivery nanosystems into the market involves an early toxicity screening in the development phase. Previously, we showed that inclusion of the polymer Eudragit (EUD) into poly(methylmethacrylate) (PMMA) nanoparticles (NP) resulted in a novel nanocarrier (PMMA-EUD) with an improved biomedical performance. The safety of this novel nanoparticulate system (PMMA-EUDNPs) was assessed in this work and compared to that of the original PMMANPs by using an integrated approach, comprising in vitro toxicity assessment and NPs physicochemical characterization in water and cell medium. For toxicity assessment several endpoints were analysed, including cell death, oxidative stress, and genotoxicity using L929 fibroblasts. PMMA-EUDNPs proved to be more hydrophobic than the original PMMANPs. Also, charge of both NPs was strongly affected by cell medium. On the other hand, the novel nanosystem was easily uptaken by L929 cells and did not display relevant in vitro cytotoxic or genotoxic effects. On the contrary, PMMANPs were less internalized in cells and proved to be genotoxic, as measured by the micronucleus assay. To conclude, our results provide initial evidence about the safety of the novel and promising PMMA-EUD nanoparticulate system, enabling its further development towards applications for drug delivery.
Peer review: yes
URI: http://hdl.handle.net/10400.18/4746
DOI: 10.1016/j.toxlet.2017.05.017
ISSN: 0378-4274
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0378427417301959
Aparece nas colecções:DGH - Artigos em revistas internacionais

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