Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/4553
Título: Proteomic and Real-Time PCR analyses of Saccharomyces cerevisiae VL3 exposed to microcystin-LR reveals a set of protein alterations transversal to several eukaryotic models
Autor: Valério, Elisabete
Campos, Alexandre
Osório, Hugo
Vasconcelos, Vitor
Palavras-chave: Apoptosis
Bacterial Toxins
DNA Repair
Gene Expression Profiling
Gene Expression Regulation, Bacterial
Marine Toxins
Osmolar Concentration
Oxidative Stress
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Species Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Two-Dimensional Difference Gel Electrophoresis
Data: 15-Mar-2016
Editora: Elsevier/ International Society on Toxinology
Citação: Toxicon. 2016 Mar 15;112:22-8. doi: 10.1016/j.toxicon.2016.01.059. Epub 2016 Jan 21
Resumo: Some of the most common toxins present in freshwater, in particular microcystins (MCs), are produced by cyanobacteria. These toxins have a negative impact on human health, being associated with episodes of acute hepatotoxicity and being considered potentially carcinogenic to humans. To date the exact mechanisms of MC-induced toxicity and tumor promotion were not completely elucidated. To get new insights underlying microcystin-LR (MCLR) molecular mechanisms of toxicity we have performed the proteomic profiling using two-dimensional electrophoresis and MALDI-TOF/TOF of Saccharomyces cerevisiae cells exposed for 4 h-1 nM and 1 μM of MCLR, and compared them to the control (cells not exposed to MCLR). We identified 14 differentially expressed proteins. The identified proteins are involved in metabolism, genotoxicity, cytotoxicity and stress response. Furthermore, we evaluated the relative expression of yeast's PP1 and PP2A genes and also of genes from the Base Excision Repair (BER) DNA-repair system, and observed that three out of the five genes analyzed displayed dose-dependent responses. Overall, the different proteins and genes affected are related to oxidative stress and apoptosis, thus reinforcing that it is probably the main mechanism of MCLR toxicity transversal to several organisms, especially at lower doses. Notwithstanding these MCLR responsive proteins could be object of further studies to evaluate their suitability as biomarkers of exposure to the toxin.
Peer review: yes
URI: http://hdl.handle.net/10400.18/4553
DOI: 10.1016/j.toxicon.2016.01.059
ISSN: 0041-0101
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0041010116300101
Aparece nas colecções:DSA - Artigos em revistas internacionais

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