Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/4542
Título: Insight into the molecular basis of Schistosoma haematobium-induced bladder cancer through urine proteomics
Autor: Bernardo, Carina
Cunha, Maria Cláudia
Santos, Júlio Henrique
da Costa, José M Correia
Brindley, Paul J
Lopes, Carlos
Amado, Francisco
Ferreira, Rita
Vitorino, Rui
Santos, Lúcio Lara
Palavras-chave: Adolescent
Adult
Aged
Animals
Carcinoma, Transitional Cell
Cell Transformation, Neoplastic
Electrophoresis, Polyacrylamide Gel
Female
Humans
Male
Middle Aged
Proteomics
Schistosoma haematobium
Schistosomiasis haematobia
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Urinary Bladder Neoplasms
Young Adult
Infecções Sistémicas e Zoonoses
Data: Ago-2016
Editora: Springer Verlag / International Society of Oncology and BioMarkers (ISOBM)
Citação: Tumour Biol. 2016 Aug;37(8):11279-87. doi: 10.1007/s13277-016-4997-y. Epub 2016 Mar 7
Resumo: Infection due to Schistosoma haematobium is carcinogenic. However, the cellular and molecular mechanisms underlying urogenital schistosomiasis (UGS)-induced carcinogenesis have not been well defined. Conceptually, early molecular detection of this phenomenon, through non-invasive procedures, seems feasible and is desirable. Previous analysis of urine collected during UGS suggests that estrogen metabolites, including depurinating adducts, may be useful for this purpose. Here, a new direction was pursued: the identification of molecular pathways and potential biomarkers in S. haematobium-induced bladder cancer by analyzing the proteome profiling of urine samples from UGS patients. GeLC-MS/MS followed by protein-protein interaction analysis indicated oxidative stress and immune defense systems responsible for microbicide activity are the most representative clusters in UGS patients. Proteins involved in immunity, negative regulation of endopeptidase activity, and inflammation were more prevalent in UGS patients with bladder cancer, whereas proteins with roles in renal system process, sensory perception, and gas and oxygen transport were more abundant in subjects with urothelial carcinoma not associated with UGS. These findings highlighted a Th2-type immune response induced by S. haematobium, which seems to be further modulated by tumorigenesis, resulting in high-grade bladder cancer characterized by an inflammatory response and complement activation alternative pathway. These findings established a starting point for the development of multimarker strategies for the early detection of UGS-induced bladder cancer.
Peer review: yes
URI: http://hdl.handle.net/10400.18/4542
DOI: 10.1007/s13277-016-4997-y
Versão do Editor: https://link.springer.com/article/10.1007%2Fs13277-016-4997-y
Aparece nas colecções:DDI - Artigos em revistas internacionais



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