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|Title: ||Diversity and phylogeny of the Helicobacter pylori outer membrane protein-encoding gene homC|
|Authors: ||Cordeiro, Rita|
|Keywords: ||Helicobacter pylori, OMP|
|Issue Date: ||Sep-2011|
|Publisher: ||Instituto Nacional de Saúde Doutor Ricardo Jorge, IP|
|Abstract: ||The genetic diversity and evolution of the homC gene was evaluated in a panel of
approximately 200 clinical and reference strains, isolated from patients from
different geographical origins and presenting different gastric diseases. PCR,
sequencing and bioinformatics analyses were used.
All the strains tested harboured a complete homC gene at a conserved locus.
Phylogenetic reconstruction of homC showed a geographical segregation, with
three predominant groups: Western, East Asian/Amerindian and African. A
similarity plot analysis suggested a conserved profile of gene segmentation, where
three segments were defined. In the first segment (5¢ end extremity), sequences
were separated according to the geographical origin of the strain. A higher level of
diversity (<50%) was observed in the middle segment, while the third segment (3¢
end extremity) was the most conserved (~90%). In the middle segment, eight
allelic variants were identified, with geographic specificity regarding the most
prevalent ones. The AI allele was predominant and exclusive of Western strains.
The AII allele was predominant in African strains and was the only allele present
in the three geographical groups. The AIV allele was predominant in East Asian/
Amerindian strains and was not observed in Western strains. The Western group
showed greater molecular distance while the sequences from the East Asian/
Amerindian group were the closest.
Overall, the regular presence of homC and its allelic variability suggest that this
gene is a good candidate to be part of the pool of H. pylori outer membrane
proteins involved in bacterial persistence.|
|Peer Reviewed: ||yes|
|Appears in Collections:||DDI - Posters/abstracts em congressos internacionais|
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