Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/3349
Título: In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells
Autor: Kiliç, Gözde
Costa, Carla
Fernández-Bertólez, Natalia
Pásaro, Eduardo
Teixeira, João Paulo
Laffon, Blanca
Valdiglesias, Vanessa
Palavras-chave: Iron Oxide Nanoparticles
Genotoxicity
Cytotoxicity
DNA Eepair
SHSY5Y cells
Genotoxicidade Ambiental
Data: 23-Out-2015
Editora: Royal Society of Chemistry
Citação: Toxicol. Res. 2016; 5: 235-47. Epub 2015 Oct 23
Resumo: Iron oxide nanoparticles (ION) have been widely used in biomedical applications, for both diagnosis and therapy, due to their unique magnetic properties. They are intensively explored in neuromedicine mostly because of their ability to cross the blood brain barrier. Hence, their potential harmful effects on neuronal cells need to be carefully assessed. The objective of this study was to evaluate the toxicity of silica-coated ION (S-ION) (10–200 μg ml−1) on human neuronal SHSY5Y cells. Alterations in the cell cycle, cell death by apoptosis or necrosis, and membrane integrity were assessed as cytotoxicity parameters. Genotoxicity was determined by a γH2AX assay, a micronucleus (MN) test, and a comet assay. Complementarily, possible effects on DNA damage repair were also analysed by means of a DNA repair competence assay. All analyses were performed in complete and serum-free cell culture media. Iron ion release from the nanoparticles was notable only in complete medium. Despite being effectively internalized by the neuronal cells, S-ION presented in general low cytotoxicity; positive results were only obtained in some assays at the highest concentrations and/or the longest exposure time tested (24 h). Genotoxicity evaluations in serum-free medium were negative for all conditions assayed; in complete medium, dose and time-dependent increase in DNA damage not related to the production of double strand breaks or chromosome loss (according to the results of the γH2AX assay and MN test), was obtained. The presence of serum slightly influenced the behaviour of S-ION; further studies to investigate the formation of a protein corona and its role in nanoparticle toxicity are necessary.
Peer review: yes
URI: http://hdl.handle.net/10400.18/3349
DOI: 10.1039/C5TX00206K
ISSN: 2045-4538
Versão do Editor: http://pubs.rsc.org/en/Content/ArticleLanding/2016/TX/C5TX00206K#!divAbstract
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