Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/3089
Título: Genotoxicity of synthetic amorphous silica nanoparticles in rats following short-term exposure. Part 1: Oral route
Autor: Tarantini, Adeline
Huet, Sylvie
Jarry, Gérard
Martine, Poul
Tavares, Ana
Vital, Nádia
Louro, Henriqueta
Silva, Maria João
Fessard, Valérie
Palavras-chave: Genotoxicidade Ambiental
Silica Nanomaterials
Comet Assay
Micronucleus Assay
Oxidative Stress
Data: Mar-2015
Editora: Wiley
Citação: Environ Mol Mutagen. 2015 Mar;56(2):218-27. doi: 10.1002/em.21935. Epub 2014 Dec 15
Resumo: Synthetic amorphous silica (SAS) in its nanosized form is now used in food applications although the potential risks for human health have not been evaluated. In this study, genotoxicity and oxidative DNA damage of two pyrogenic (NM-202 and 203) and two precipitated (NM-200 and -201) nanosized SAS were investigated in vivo in rats following oral exposure. Male Sprague Dawley rats were exposed to 5, 10, or 20 mg/kg b.w./day for three days by gavage. DNA strand breaks and oxidative DNA damage were investigated in seven tissues (blood, bone marrow from femur, liver,spleen, kidney, duodenum, and colon) with the alkaline and the (Fpg)-modified comet assays, respectively. Concomitantly, chromosomal damage was investigated in bone marrow and in colon with the micronucleus assay. Additionally, malondialdehyde (MDA), a lipid peroxidation marker, was measured in plasma. When required, a histopathological examination was also conducted. The results showed neither obvious DNA strand breaks nor oxidative damage with the comet assay, irrespective of the dose and the organ investigated. Similarly, no increases in chromosome damage in bone marrow or lipid peroxidation in plasma were detected. However, although the response was not dose-dependent, a weak increase in the percentage of micronucleated cells was observed in the colon of rats treated with the two pyrogenic SAS at the lowest dose (5 mg/kg b.w./day). Additional data are required to confirm this result, considering in particular, the role of agglomeration/aggregation of SAS NMs in their uptake by intestinal cells.
Peer review: yes
URI: http://hdl.handle.net/10400.18/3089
DOI: 10.1002/em.21935
ISSN: 0893-6692
Versão do Editor: http://onlinelibrary.wiley.com/doi/10.1002/em.21935/abstract;jsessionid=D754FB6D0A9F4EED8476E674BCEABA76.f04t01
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