Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/3044
Título: Evaluation of combined cytotoxic effects of ochratoxin a and fumonisin B1 in human liver and renal cells
Autor: Tavares, Ana
Mendonça, I.
Alvito, Paula
Louro, H.
Silva, M.J.
Loureiro, S.
Palavras-chave: Segurança Alimentar
Toxicologia
Avaliação de Risco
Saúde Humana
Data: Abr-2015
Resumo: Mycotoxins are fungal food contaminants with potential to cause severe acute and chronic conditions1. Therefore, food contamination with mycotoxins such as ochratoxin A (OTA) and fumonisin B1 (FB1) causes great concern. Previous studies addressed the co-occurrence of these toxins in foods2, however there is little knowledge on their combined cytotoxic effects. In the present study we aimed to evaluate the cytotoxic effects of mixtures of OTA and FB1 in two human-derived cell lines. For this purpose, neutral red and MTT assays were performed. In HepG2 cells, OTA caused a significant decrease in cell viability after 24h exposure (above 10 µM; p<0.001), with an IC50 of 27.5 µM. However, no significant cytotoxic effects were observed after 24h exposure with FB1. When in mixture, both mycotoxins caused a non-significant decrease in the viability of HepG2 cells compared to the effects of the FB1 individually. In HK-2, OTA caused a significant decrease in cells viability after 24h exposure (above 5 µM; p<0.001), with an IC50 of 7.5 µM. Also, exposure to FB1 during 24h caused significant cytotoxic effects (above 320 µM; p<0.001), with an IC50 of 1.1 mM. The mixture of both toxins was significantly different from all the respective individual treatments of OTA and FB1 (p< 0.006). After modelling these data with the Concentration Addition conceptual model, there was a significant deviation for the dose level pattern, depicting a synergism at low dose levels of both mycotoxins, but changing to antagonism at higher doses. Therefore, considering the lower doses as the more relevant and potential to occur, this results highlight the importance of studies like this, since an increase in toxicity was observed, being higher than expected. These results agree with those presented by Creppy et al. (2004) with synergistic effects between OTA and FB1 in Vero cells3. Further work must be performed to disclose which genetoxic effects these toxins might cause to these cell lines
Peer review: yes
URI: http://hdl.handle.net/10400.18/3044
Aparece nas colecções:DAN - Posters/abstracts em congressos internacionais



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