Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/3042
Título: Interactions between dietary toxins and gut mucosa: patulin effects on the structure and function of intestinal and immune cells
Autor: Assunção, Ricardo
Alvito, Paula
Kleiveland, C.
Lea, T.
Palavras-chave: Segurança Alimentar
Toxicologia
Avaliação de Risco
Saúde Humana
Data: Mar-2015
Resumo: The intestinal mucosa is the first biological barrier encountered by natural toxins, and consequently, it could be exposed to high amounts of dietary toxins. Mycotoxins are a wide group of fungal secondary metabolites that exert toxic effects in humans. Patulin (PAT), a mycotoxin produced by Penicillium spp. during fruit spoilage, is a major concern with regard to children's health because its ingestion could result in severe acute and chronic toxicity3,4. This study aimed to evaluate the effect of PAT on the structure and function of the gut mucosa and its interactions with intestinal and immune cells. Several methodologies were applied in order to characterize the effects of PAT on intestinal cell model (Caco-2), human peripheral blood lymphocytes (PBL) and human blood monocyte-derived dendritic cells (DCs). As evaluated by an MTT assay, PAT exposure reduced the viability of Caco-2 cells, mainly for concentrations above 12 µM. The integrity of the Caco-2 monolayer was affected after exposure to PAT, as demonstrated by a decrease in transepithelial electrical resistance values, becoming more pronounced for 50 µM of PAT. At this concentration, using SDS-PAGE and immunoblotting, no effects were detected on the expression levels of the tight junction proteins occludin, claudin-1 and claudin-3. However, the expression of zonula occludens-1 and myosin light chain (MLC) declined after 24h of exposure. The phospho-MLC increased after 24h of exposure to 50 µM of PAT. T cell proliferation was evaluated by thymidine incorporation assay, incubating PBL with soluble anti-CD3 antibodies. T cell proliferation was highly sensitive to PAT with the major effects for concentrations above 10 nM of PAT. Surprisingly, the same conditions did not affect the maturation of DCs for PAT concentrations between 0.5 nM and 100 nM. These studies provide more and new information about how patulin may affect the intestinal epithelium and mucosal immune responsiveness.
Peer review: yes
URI: http://hdl.handle.net/10400.18/3042
Aparece nas colecções:DAN - Posters/abstracts em congressos internacionais



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