Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/2956
Título: Antimicrobial resistance determinants in Staphylococcus spp. recovered from birds of prey in Portugal
Autor: Sousa, Margarida
Silva, Nuno
Igrejas, Gilberto
Silva, Filipe
Sargo, Roberto
Alegria, Nuno
Benito, Daniel
Gómez, Paula
Lozano, Carmen
Gómez-Sanz, Elena
Torres, Carmen
Caniça, Manuela
Poeta, Patrícia
Palavras-chave: Resistência aos Antibióticos
Coagulase-negative staphylococci
Birds of Prey
Ica Operon
S. aureus
S. epidermidis
Data: Jun-2014
Editora: Elsevier
Citação: Vet Microbiol. 2014 Jul 16;171(3-4):436-40. doi: 10.1016/j.vetmic.2014.02.034. Epub 2014 Mar 3
Resumo: Antibiotic resistance among wild animals represent an emerging public health concern. The objective of this study was to analyze the staphylococcal nasal microbiota in birds of prey and their content in antimicrobial resistance determinants. Nasal samples from 16 birds of prey were collected, swabs were dipped and incubated into BHI broth [6.5% NaCl] and later seeded on manitol salt agar and oxacillin-resistance screening agar base media. Staphylococcal colonies were isolated from both media and were identified by biochemical and molecular methods. Susceptibility testing to 18 antimicrobial agents was performed by disk-diffusion method. Six of the 16 tested animals carried staphylococci (37.5%) and 7 isolates of the following species were recovered: Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Staphylococcus sciuri rodentium, Staphylococcus cohnii urealitycum, and Staphylococcus gallinarum. The S. aureus isolate was penicillin-resistant (with blaZ gene) but methicillin-susceptible and was ascribed to spa-type t012, sequence-type ST30 and agr-type III. The S. epidermidis isolate carried blaZ, mecA, mrs(A/B), mphC, tet(K), drfA, and fusC genes, ica operon, and was typed as ST35. The genes ant6′-Ia, tet(K), tet(L), dfrG, cat221, cat194, and cat223 were detected in S. saprophyticus or S. gallinarum isolates. Birds of prey seem to be a natural reservoir of S. aureus and coagulase-negative staphylococci resistant to multiple antibiotics. Due to the convergence between habitats, the contact between wildlife, other animals and humans is now more common and this involves an increased possibility of interchange of these microorganisms in the different ecosystems.
Peer review: yes
URI: http://hdl.handle.net/10400.18/2956
DOI: 10.1016/j.vetmic.2014.02.034
ISSN: 0378-1135
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0378113514001242
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