Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/2947
Título: Isolation and molecular characterization of Toxoplasma gondii isolated from pigeons and stray cats in Lisbon, Portugal
Autor: Vilares, Anabela
Gargaté, Maria João
Ferreira, Idalina
Martins, Susana
Júlio, Cláudia
Waap, Helga
Ângelo, Helena
Gomes, João Paulo
Palavras-chave: Toxoplasma gondii
Cats
Pigeon
Genotype
Infecções Sistémicas e Zoonoses
Genotipagem
Data: 15-Out-2014
Editora: Elsevier/ American Association of Veterinary Parasitologists, the European Veterinary Parasitology College, World Association for the Advancement of Veterinary Parasitology
Citação: Vet Parasitol. 2014 Oct 15;205(3-4):506-11. doi: 10.1016/j.vetpar.2014.08.006. Epub 2014 Aug 26
Resumo: Cats and pigeons are important factors in the epidemiology of Toxoplasma gondii as felids are the only definitive hosts that can excrete environmentally resistant oocysts, and pigeons share the same places of cats and humans constituting a good model and indicator of the ground field contamination. We aimed to study the virulence and genotypes of T. gondii isolated from pigeons and stray cats in Lisbon, Portugal. Fresh samples of brain from 41 pigeons and 164 cats revealing antibodies to T. gondii were inoculated in mice. Three isolates (one isolated from a cat and two isolated from pigeons) were virulent in the mouse model. Sag2-based genotyping of T. gondii was achieved in 70.7% (29/41) of samples isolated from pigeons (26 samples were type II, two were type III, and one strain was type I). From the cat brain samples, 50% (82/164) yielded Sag2 positive results, where 72 belonged to genotype II and 10 were no type III (it was not possible to discriminate between type I and II). Further genotyping was obtained by multiplex PCR of 5 microsatellites (TUB2, TgM-A, W35, B17, B18), allowing the identification of two recombinant strains that had been previously identified as type II by Sag2 amplification (one isolated from cat brain and the other from pigeon brain). This is the first evidence of recombinant strains circulating in Portugal and the first report of T. gondii genotyping from cats in this country. This study also highlights the importance of environmental contamination in the synanthropic cycle constituting a potential source of human infection.
Peer review: yes
URI: http://hdl.handle.net/10400.18/2947
DOI: 10.1016/j.vetpar.2014.08.006
ISSN: 0304-4017
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0304401714004439
Aparece nas colecções:DDI - Artigos em revistas internacionais

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