Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/2919
Título: Human, food and animal Campylobacter spp. isolated in Portugal: high genetic diversity and antibiotic resistance rates
Autor: Duarte, A.
Santos, A.
Manageiro, V.
Martins, A.
Fraqueza, M.J.
Caniça, Manuela
Domingues, F.
Oleastro, M.
Palavras-chave: Campylobacter
Genetic Diversity
Antibiotic Susceptibility
Infecções Gastrointestinais
Portugal
Data: Out-2014
Editora: Elsevier/ International Society of Chemotherapy
Citação: Int J Antimicrob Agents. 2014 Oct;44(4):306-13. doi: 10.1016/j.ijantimicag.2014.06.012. Epub 2014 Jul 26
Resumo: Infections by Campylobacter jejuni and Campylobacter coli are considered the major cause of bacterial gastroenteritis in humans, with food being the main source of infection. In this study, a total of 196 Campylobacter strains (125 isolates from humans, 39 from retail food and 32 from food animal sources) isolated in Portugal between 2009 and 2012 were characterised by multilocus sequence typing (MLST) and flaA short variable region (SVR) typing. Susceptibility to six antibiotics as well as the mechanisms underlying antibiotic resistance phenotypes was also studied. Based on MLST typing, C. coli strains were genetically more conserved, with a predominant clonal complex (CC828), than C. jejuni strains. In contrast, C. coli isolates were genetically more variable than C. jejuni with regard to flaA-SVR typing. A high rate of resistance was observed for quinolones (100% to nalidixic acid, >90% to ciprofloxacin) and, in general, resistance was more common among C. coli, especially for erythromycin (40.2% vs. 6.7%). In addition, most isolates (86%) were resistant to multiple antimicrobial families. Besides the expected point mutations associated with antibiotic resistance, detected polymorphisms in the cmeABC locus likely play a role in the multiresistant phenotype. This study provides for the first time an overview of the genetic diversity of Campylobacter strains from Portugal. It also shows a worrying antibiotic multiresistance rate and the emergence of Campylobacter strains resistant to antibiotics of human use.
Peer review: yes
URI: http://hdl.handle.net/10400.18/2919
DOI: 10.1016/j.ijantimicag.2014.06.012
ISSN: 0924-8579
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0924857914002076
Aparece nas colecções:DDI - Artigos em revistas internacionais

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
1-s2.0-S0924857914002076-main.pdf502,67 kBAdobe PDFVer/Abrir    Acesso Restrito. Solicitar cópia ao autor!


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.