Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.18/2914
Título: Identification of nontuberculous mycobacteria by partial gene sequence and public databases
Autor: Joâo, Inês
Cristovão, Paula
Antunes, Liliana
Nunes, Baltazar
Jordão, Luísa
Palavras-chave: Nontuberculous Mycobacteria (NTM)
Public Databases
hsp65
16S rRNA
Infecções Respiratórias
Estados de Saúde e de Doença
Data: 14-Mai-2014
Editora: Elsevier
Citação: Int J Mycobacteriol. 2014;3(2):144-51.doi:10.1016/j.ijmyco.2014.04.001
Resumo: Background: Nontuberculous mycobacteria (NTM) are a heterogeneous group of microorganisms with distinct clinical relevance. The treatment of NTM infections depends significantly upon the crucial identification of species at this level. The steady increase of mycobacteria species, the use of time-consuming techniques and the lack of standardized identification methods makes the achievement of this goal a demanding challenge. Additionally, inaccurate diagnosis can lead to therapeutic approaches consistent with Mycobacterium tuberculosis infection that are useless. In the present study, the performance of public databases in the accurate identification of NTM by sequence analysis of 16S rRNA and hsp65 genes were evaluated and compared. An algorithm is proposed to achieve an accurate classification of NTM in the geographic region of Portugal (Western Europe). Methods: Partial sequencing of 16S rRNA and hsp65 genes of 22 reference strains and 54 clinical isolates was performed. The resulting sequences were analysed by public web databases since their performance is evaluated statistically. The phenotypic characteristics of the isolates were also evaluated. Results: The use of commercial kits allowed the accurate identification of 57.4% of the clinical isolates. This result was improved either by the use of 16S rRNA (75.9%) and hsp65 (88.9%) genes analysis alone or combined (96.3%. Conclusions: Analysis of 16S rRNA gene alone is insufficient for the accurate identification of NTM. A stepwise algorithm combining 16S rRNA and hsp65 gene analysis by multiple public databases is proposed to identify NTM at the species’ level.
Peer review: yes
URI: http://hdl.handle.net/10400.18/2914
DOI: 10.1016/j.ijmyco.2014.04.001
ISSN: 2212-5531
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S2212553114000314
Aparece nas colecções:DAN - Artigos em revistas internacionais
DDI - Artigos em revistas internacionais

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