Repositório Científico do Instituto Nacional de Saúde >
Departamento de Doenças Infecciosas >
DDI - Artigos em revistas internacionais >
Please use this identifier to cite or link to this item:
|Title: ||Isolates from hospital environments are the most virulent of the Candida parapsilosis complex|
|Authors: ||Sabino, Raquel|
|Keywords: ||Candida parapsilosis|
Infecções Sistémicas e Zoonoses
|Issue Date: ||8-Aug-2011|
|Publisher: ||BioMed Central|
|Citation: ||BMC Microbiol. 2011 Aug 8;11:180|
|Abstract: ||Background: Candida parapsilosis is frequently isolated from hospital environments, like air and surfaces, and
causes serious nosocomial infections. Molecular studies provided evidence of great genetic diversity within the C.
parapsilosis species complex but, despite their growing importance as pathogens, little is known about their
potential to cause disease, particularly their interactions with phagocytes. In this study, clinical and environmental
C. parapsilosis isolates, and strains of the related species C. orthopsilosis and C. metapsilosis were assayed for their
ability to induce macrophage cytotocixity and secretion of the pro-inflammatory cytokine TNF-a, to produce
pseudo-hyphae and to secrete hydrolytic enzymes.
Results: Environmental C. parapsilosis isolates caused a statistically significant (p = 0.0002) higher cell damage
compared with the clinical strains, while C. orthopsilosis and C. metapsilosis were less cytotoxic. On the other hand,
clinical isolates induced a higher TNF-a production compared with environmental strains (p < 0.0001). Whereas the
amount of TNF-a produced in response to C. orthopsilosis strains was similar to the obtained with C. parapsilosis
environmental isolates, it was lower for C. metapsilosis strains. No correlation between pseudo-hyphae formation or
proteolytic enzymes secretion and macrophage death was detected (p > 0.05). However, a positive correlation
between pseudo-hyphae formation and TNF-a secretion was observed (p = 0.0119).
Conclusions: We show that environmental C. parapsilosis strains are more resistant to phagocytic host defences
than bloodstream isolates, being potentially more deleterious in the course of infection than strains from a clinical
source. Thus, active environmental surveillance and application of strict cleaning procedures should be
implemented in order to prevent cross-infection and hospital outbreaks.|
|Peer Reviewed: ||yes|
|Publisher version: ||http://www.biomedcentral.com/1471-2180/11/180|
|Appears in Collections:||DDI - Artigos em revistas internacionais|
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.